PBS-Tween BSA for 300ml [0.05% Tween 20, 5%BSA, 0.05% azide, PBS]

PBS-Tween BSA for 300ml [0.05% Tween 20, 5%BSA, 0.05% azide, PBS] Mix on magnetic stirrer: 300ml PBS-Tween 15g BSA {Sigma, No.:A3059-100G, Albumin, bovine serum, Fraction V} 1.5ml Sodium azide (from 10% NaN3 stock solution) Filtrate through an 0.45um filter Aliquot 50ml/falcon Store at 4°C