Gel Running Buffer or 1x TBE (Tris-Borate-EDTA) Buffer Recipes

1x TBE (Tris-Borate-EDTA) Buffer

90 mM Tris

• tris(hydroxymethyl)aminomethane

• Tris base; FW=121.14

90 mM Boric acid

• Boric acid; FW= 61.83

2 mM EDTA

• ethylenediaminetetraacetic acid; FW= 292.24

• ethylenediaminetetraacetic acid dihydrate

• ethylenediaminetetraacetic acid disodium salt, or disodium ethylenediaminetetraacetic acid; FW= 372.24

This typically results in a solution close to pH 8.3.

This is often prepared as a 5x TBE stock solution and diluted to 1x or 0.5x as needed.