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Wednesday, 6 June 2007

How to Make 1xPBS (Phosphate Buffered Saline)?

1x PBS (Phosphate Buffered Saline)

4.3 mM Sodium Phosphate, Dibasic

  • Na2HPO4; FW = 141.96

137 mM Sodium Chloride

  • NaCl; FW = 58.44

2.7 mM Potassium Chloride

  • KCl; FW = 74.56

1.4 mM Potassium Phosphate, Monobasic

  • KH2PO4; FW = 136.09

Add hydrochloric acid (HCl) to bring the pH to 7.4.

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