Used for storing and diluting DNA or RNA.
10 mM Tris
Can be made either by weighing calculated mass of each powder and dissolving in ultrapure water, or by diluting typical laboratory stock solutions of 1 M Tris and 0.5 M EDTA.
This is often prepared as a 10x TE stock solution and diluted to 1x as needed.
How to make PBS
10 mM Tris
- tris(hydroxymethyl)aminomethane
- Tris base; FW=121.14
- Tris-HCl acid; FW 157.61
- ethylenediaminetetraacetic acid; FW= 292.24
- ethylenediaminetetraacetic acid dihydrate
- ethylenediaminetetraacetic acid disodium salt or disodium ethylenediaminetetraacetic acid; FW= 372.24
Can be made either by weighing calculated mass of each powder and dissolving in ultrapure water, or by diluting typical laboratory stock solutions of 1 M Tris and 0.5 M EDTA.
This is often prepared as a 10x TE stock solution and diluted to 1x as needed.
How to make PBS
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