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Tuesday, 28 August 2007

New RNAi Screening LentiExpress(TM) Technology

Sigma-Aldrich Announces LentiExpress(TM) Technology for Faster and Easier High-Throughput RNAi Screening

PR Newswire

Novel viral delivery method saves significant time and costs with a ready-to-go process for whole-kinome screens.

Novel viral delivery method saves significant time and costs with a ready-to-go process for whole-kinome screens. ST. LOUIS, Aug. 28 /PRNewswire-FirstCall/ -- Sigma-Aldrich (Nasdaq: SIAL) is pleased to introduce the LentiExpress shRNA-based system ( for rapid high-throughput RNAi screening with minimal reagent preparation or optimization required. The addition of this technology to the MISSION(R) shRNA platform pairs the benefits of lentiviral-based shRNA with a simple streamlined protocol. The bottleneck for researchers is the time and expense required to generate viral particles and devise a robust screening strategy. With the MISSION LentiExpress technology, they can eliminate reagent preparation with a sophisticated yet simple method to perform complex screens. An Optimization Plate enables researchers to quickly optimize the system for their particular cell line. This optimization process is uniquely simple compared to cumbersome transfection optimization methods that exist for siRNA-based screens. A researcher simply adds the desired number of cells to each well, continues with optional selection and/or addition of small molecules (for example, pharmaceutical compounds), and then proceeds directly to the desired assay for gene silencing or loss-of-function.

The LentiExpress format with the Human Kinase shRNA collection of The RNAi Consortium (TRC) enables rapid human kinome RNAi screening. The MISSION LentiExpress Human Kinase shRNA set consists of 3,109 individual pre-arrayed lentiviral clones harboring shRNA sequences that target 673 human kinase genes for gene silencing. "Because we created the product to be ready-to-go single-use assays, researchers now have access to a fast and affordable means to utilize RNAi in whole-kinome screens. Lentiviral-based shRNA is also known for the benefit of being able to deliver RNAi effector molecules to a wide variety of cells including primary and non-transfectable cells lines," said Dr. Edward Weinstein, Manager of Functional Genomics Operations at Sigma-Aldrich. "Another benefit is stable long-term knockdown compared to transient transfection based siRNA options." "Sigma-Aldrich researchers have performed proof-of-principle LentiExpress screens examining which genes might play a role in the modulation of a well-known cancer chemotherapy drug," said Dr. David Smoller, President of the Sigma-Aldrich Research Biotech business unit. "The screen resulted in hits already validated by the literature as well as additional hits that could be further investigated as prognostic markers for susceptibility to treatment. Both internal and external validations of the methodology have proven the utility of this exciting technology." The LentiExpress technology complements the already robust MISSION shRNA product line which includes libraries, gene family sets, and gene target sets for The RNAi Consortium lentiviral-based shRNA collection targeting the human and mouse genomes. The product portfolio includes a viral packaging system, a variety of controls, as well as the unique ability to offer any or all of the collection in a choice of glycerol stock, purified DNA or viral particle format.


Sigma-Aldrich's complete RNAi platform also includes the MISSION siRNA libraries, mRNA detection reagents, antibodies and AQUA peptides for protein level detection as well as an entire workflow of supporting reagents (cell culture, cell biology compounds and assays, transfection reagents, and much more). For more information, visit About Sigma-Aldrich: Sigma-Aldrich is a leading Life Science and High Technology company. Its biochemical and organic chemical products and kits are used in scientific and genomic research, biotechnology, pharmaceutical development, the diagnosis of disease and as key components in pharmaceutical and other high technology manufacturing. Sigma-Aldrich has customers in Life Science companies, university and government institutions, hospitals, and in industry. Over one million scientists and technologists use its products. Sigma-Aldrich operates in 36 countries and has 7,700 employees providing excellent service worldwide. Sigma-Aldrich is committed to Accelerating Customer Success through Leadership in Life Science, High Technology and Service.

For more information about Sigma-Aldrich, please visit its award-winning Web site at About TRC: TRC is comprised of principal investigators from world-class academic research institutions (Massachusetts Institute of Technology, Harvard Medical School, the Broad Institute, Whitehead Institute for Biomedical Research, Dana-Farber Cancer Institute, Massachusetts General Hospital, Washington University, Columbia University, and Academia Sinica) as well as corporate sponsoring institutions (Sigma-Aldrich, Novartis, Eli Lilly, and Bristol-Myers Squibb). As a scientific collaborator and distribution partner through agreement with MIT, Sigma-Aldrich is working with TRC to provide the scientific community with RNAi tools for functional genomics research specifically for gene function discovery and the study of disease. The MISSION TRC shRNA clone libraries comprise a comprehensive collection of over 150,000 pre-cloned lentiviral-based shRNA vector constructs targeting more than 15,000 human genes (MISSION TRC-Hs1.0) and 15,000 mouse genes (MISSION TRC-Mm1.0).

Design and development of the TRC libraries is being led by the Broad Institute of the Massachusetts Institute of Technology (MIT) and Harvard. For more information about MISSION shRNA clone collections, please visit us online at Cautionary Statement: This release contains forward-looking statements relating to future performance, goals, strategic actions and initiatives and similar intentions and beliefs and other statements regarding the Companies' expectations, goals, beliefs, intentions and the like, which involve assumptions regarding the Companies' operations and conditions in the markets the Companies serve. The Companies do not undertake any obligation to update these forward-looking statements.

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Monday, 27 August 2007

Protocols for Neural Cell Culture

Protocols for Neural Cell Culture

* Publisher: Humana Press
* Number Of Pages: 384
* Publication Date: 2001-02-15
* Sales Rank: 1339161
* ISBN / ASIN: 0896039021
* EAN: 9780896039025
* Binding: Plastic Comb
* Manufacturer: Humana Press
* Studio: Humana Press

Sergey Fedoroff and Arleen Richardson extensively revise, update, and expand their best-selling and highly praised collection of readily reproducible neural tissue culture protocols. This 3rd edition adds 11 new chapters describing important new procedures for the isolation, growth, and characterization of neural stem cells and for the manipulation of glial progenitor cells, as well as are essential procedures for hippocampal and microglial slice cultures. Protocols for Neural Cell Culture: Third Edition is a richly augmented updating of the tried and tested laboratory procedures that have made earlier editions an indispensable reference and guide to neural cell culture and its disorders.


Human Papillomaviruses Methods and Protocols (Methods in Molecular Medicine)

Human Papillomaviruses: Methods and Protocols (Methods in Molecular Medicine)

Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

  • Publisher: Humana Press
  • Number Of Pages: 512
  • Publication Date: 2005-09-01
  • Sales Rank: 2030193
  • ISBN / ASIN: 1588293734
  • EAN: 9781588293732
  • Binding: Hardcover
  • Manufacturer: Humana Press
  • Studio: Humana Press
  • Book Description:
  • Download
  • English Grammar - A University Course

    This comprehensive and up-to-date descriptive grammar is a complete course for first degree and postgraduate students of English, especially those whose native language is not English. It is also used as a reference book providing the linguistic basis for courses and projects on translation, contrastive linguistics, stylistics, reading and discourse studies.


    Oxford Dictionary

    Oxford Dictionary

    Online Neuroscience Lectures

    Online Neuroscience Lectures
    · Krieger Mind/Brain Institute at Johns Hopkins
    1. Cortical Self-Organization and Perceptual Learning – Mike Kilgard (PowerPoint slides)
    2. Behavioral and neural correlates of semantic processing in rhesus macaques – Yale E. Cohen
    3. Neuronal Mechanisms of Visual Categorization and Object Recognition – David Freedman
    · McCuistion Program on PBS (Appropriate for non-scientific audiences)
    1. Discoveries and Hope for Brain Health DSL/Cable Dial-up
    2. A Lifetime of Brain Building DSL/Cable Dial-up
    · MIT Lectures (requires Real Player to view)
    1. Neurobiology of Memory: How Do We Acquire, Consolidate and Recall Memory – Susumu
    2. The Changing Brain - Mark Bear
    3. Architecture of the Brain - Elly Nedivi
    4. Vision: Challenges and Prospects - Pawan Sinha
    5. 2002 Nobel Prize in Physiology Nobel Lecture - Robert Horvitz
    6. Cognitive Control: Understanding the Brain's Executive - Earl K. Miller
    7. Words and Rules: The Ingredients of Language - Steven Pinker
    · Conference on Brain Network Dynamics - 2007
    · The Storage and Persistence of Memory – Eric Kandel
    · Dana Foundation (Appropriate for non-scientific audiences)
    · UCLA Joint Seminars in Neuroscience – Eight years of archived basic and clinical neuroscience talks
    1. "Substrates of Rapid Plasticity in Developing Cortex" Michael P. Stryker, Ph.D.
    2. "Synaptic Mechanisms for Experience-Dependent Plasticity in Rat Somatosensory Cortex" Dan E. Feldman
    · Rice University: Neuroscience Lectures (requires Real Player to view)
    1. Cortical Dynamics and Visual Perception" - Charles D. Gilbert
    2. "Attention as an Organ System” - Michael I. Posner
    3. "The Hippocampus and Declarative Memory: Cognitive Mechanisms and Neural Codes" - Howard Eichenbaum
    4. Brain Overview Michael P. Stryker and Huda Zoghbi
    · NIH Video Archive: Neuroscience Seminar Series (requires Real Player to view)
    1. Plasticity of the Somatosensory System in Mature and Developing Primates - Jon H. Kaas
    2. Control of Synapse Number and Strength in Developing Cortical Networks - Gina Turrigiano
    3. Postsynaptic Mechanisms of Plasticity - Morgan Sheng
    4. Stability and Plasticity in Primate Visual Development - J. Anthony Movshon
    5. Molecular Control of Cortical Connectivity - Anirvan Ghosh
    6. Functions of Electrical Coupling in Inhibitory Networks of the Neocortex - Barry Connors
    7. Prefrontal Cortex and the Neural Basis of Cognition - Earl Miller
    8. Basal Ganglia and Cerebellar 'Loops' within the Cerebral Cortex: Motor and Cognitive Circuits - Peter Strick
    9. Diversity of GABAergic Neurons and Spike Timing in the Cerebral Cortex - Peter Somogyi
    10. Reward Processing in Primate Basal Ganglia and Frontal Cortex - Wolfram Schultz
    11. A Thorny Path to Memories: Dendritic Spines, Neurotrophins and their Role in Synaptic Plasticity - Tobias Bonhoeffer
    12. Brain Waves and Immune Genes in Brain Wiring During Development - Carla Shatz
    13. Neural Mechanisms of Human Cognition: Insights from Brain Imaging Studies - Leslie G. Ungerleider
    14. The Dynamic Brain - Terry Sejnowski
    15. Homeostatic Regulation of Synaptic Structure and Function - Graeme Davis
    16. Nobel Laureates, 2000 - 1) Molecular Biology of Memory and Its Disorders: Some Societal Implications 2) The Neurobiology of Slow Synaptic Transmission - Eric R. Kandel and Paul Greengard
    17. The Ingredients of Language - Steven Pinker
    18. Know Stroke - Know the Signs - Act in Time
    · Others
    1. Picower Institute Inaugural Symposium: The Future of the Brain
    2. Vega Science Trust
    3. Magnetic brain stimulation: what can it tell us about brain function? – Alan Cowey FRS
    4. Talk on how brain controls body
    5. Royal Society

    Saturday, 25 August 2007

    ebook-Reactivation of the Cell Cycle in Terminally Differentiated Cells (Molecular Biology Intelligence Unit, 17)

    Reactivation of the Cell Cycle in Terminally Differentiated Cells (Molecular Biology Intelligence Unit, 17)

    Reactivation of the Cell Cycle in Terminally Differentiated Cells (Molecular Biology Intelligence Unit, 17)

  • Publisher: Springer
  • Of Pages:number 200
  • Date:publication 2003-02-28
  • Rank:sales 4356609
  • ISBN / ASIN: 0306474239
  • EAN: 9780306474231
  • Binding: Hardcover
  • Manufacturer: Springer
  • Studio: Springer
  • Description: book
  • Link
  • Wednesday, 22 August 2007

    Invitrogen now offers validated GPCR cells by the plate


    Invitrogen now offers validated GPCR cells by the plate

    Division Arrest technology for GPCRs allows you to:

    Use frozen cells as ordinary and cost-effective assay reagents for screening
    Decouple labor- and time-intensive live cell production from your screening
    Obtain response profiles similar to those of growing cells, thus ensuring the correct pharmacological profile
    Remove variability caused by cell division during an assay

    GeneBLAzer® Division Arrested (DA) cells are available in two convenient configurations - an assay kit (includes cells and enough substrate to analyze one 384-well plate) or a tube of cells sufficient to analyze ten 384-well plates (substrate available separately).

    See the current list of GeneBLAzer® Division Arrested GPCR cells.

    For information about our latest GPCR assay technology coming soon, please see


    Science Protocols