International Scholarships and Financial Aid

Wednesday, 24 October 2007

analyze gene expression or DNA methylation

Do you knock down genes with siRNA, analyze gene expression or DNA methylation, or study miRNAs? We understand the major challenges that have to be overcome.

 

Our sample and assay technologies ensure that you obtain the reliable, high-quality data you need. From sample collection and stabilization to analyte purification and analysis - we’ve got it covered.

 

For more details, visit our application pages:

 

You can also find out more in our new brochure Analyzing Gene Expression and Regulation.

Monday, 22 October 2007

PCR.ppt- Microsoft PowerPoint

[PDF]

Microsoft PowerPoint - PCR.ppt

File Format: PDF/Adobe Acrobat - View as HTML
Nucleotides. Primers. polymerase. PCR is DNA replication in a test tube ... Reverse trancriptase PCR. Use mRNA as a template. Isolated cDNA clones ...
learn.sdstate.edu/chend/Courses/2006.Fall/Math592.Bioinformatics/PCR.pdf -

Friday, 19 October 2007

ARK-Genomics conference 2008: 3rd International Symposiumon Animal Functional Genomics (ISAFG)

ARK-Genomics conference 2008: 3rd International Symposium on Animal Functional Genomics (ISAFG)

Dates:
April 7th – 9th 2008

Venue:
Edinburgh International Conference Centre (EICC), Morrison Street, Edinburgh, UK

Official Conference Website:
http://ebrc-launch.org/ISAFG/index.html
(updated information about agenda, registration, abstract submission, accommodation and venue)

Registration and Abstract Submission will open on Friday, October 12th 2007
Reduced rates for PhD and first year post docs available.
Limited number of travel bursaries for young researchers available.

Highlights:
The 3rd International Symposium on Animal Functional Genomics (ISAFG) will present opportunities to take stock of the current European and International position in animal functional genomics, identifying areas for collaborations and the development of new resources.

Increased exposure through the bringing together of two important conference series: 
1st European Farm Animal Functional Genomics Conference (ARK-Genomics) and
2nd International Symposium on Animal Functional Genomics (Michigan State University)

Themes:
The conference will be structured around the following themes:
“Genetics and QTLs”

“Bioinformatics and Data Mining”

“Animal Health”

“Functional Genomics meets Physiology”

“Animal Genomics in Industry”

“Proteomics”

“Systems Biology”

The ISAFG event will run in parallel with the launch of the EBRC, a new world class animal bioscience centre.
The keynote lectures by Professor John Quackenbush and Professor Michel Georges will highlight functional genomics and its impact on Systems Biology.

Additionally there will be 2 open sessions, a poster session with cheese &wine as well as a conference dinner.
As before, there will be plenty of opportunities for networking.


More information about ARK-Genomics, the collaborative functional genomics research centre can be found at:

http://www.ark-genomics.org/

 

Wednesday, 17 October 2007

RNA In Situ Hybridization Protocol

96-well RNA In Situ Hybridization Protocol - http://www.fruitfly.org/about/methods/RNAinsitu.html

96-well RNA In Situ Hybridization Protocol. Probe Preparation, Cell Inoculation, PCR, RNA Probe Preparation, etc, Very In-depth Protocol and Method Conditions. Berkeley Drosophila Genome Project.

http://www.fruitfly.org/about/methods/RNAinsitu.html

Milestones in DNA Technologies

Milestones in DNA Technologies

A collaboration from Nature, Nature Methods and Nature Reviews Genetics, Milestones in DNA Technologies will focus on ground-breaking technologies and advances in the analysis of DNA. It will contain articles from the participating journals and original commentaries written by personalities who have witnessed the unfolding, or consequences, of these milestone achievements.

Free print copies available!
Click here to request your free print copy.

Sunday, 14 October 2007

Climate Scientists, Gore Share Nobel Peace Prize

Climate Scientists, Gore Share Nobel Peace Prize
Committee honors efforts to spread global awareness of climate change
http://sciencenow.sciencemag.org/cgi/content/full/2007/1012/1?etoc

Friday, 12 October 2007

Neuron SFN Satellite Meeting: Register Now!

Only 21 days until the meeting... Register to attend and Submit your Abstract today!

Neurons on the Map (just preceding the SfN meeting)
November 1, 2007
San Diego, CA

For the complete meeting details, visit http://www.neuron-meeting.com.

If you have any questions regarding registration, please contact Charlotte Wilkins c.wilkins@elsevier.com.

Viral Metagenomics WEBINAR

Viral Metagenomics WEBINAR

Join an online seminar on 24 October 2007 entitled "A Viral Metagenomics Study of Honey Bee Colony Collapse Disorder" presented by two eminent authors of a recently published Science paper.

Submit your questions LIVE to the experts during the webinar!

Date: Wednesday, October 24, 2007
Time: 12:00 noon EDT; 9:00 a.m. PDT ; 5 p.m. GMT
Duration: 1 hour

Register now!
For more information and complimentary registration visit:
www.sciencemag.org/webinar


Colony collapse disorder (CCD) among honey bee populations in the United States has resulted in the loss of between 50% and 90% of hive colonies. Previous studies have pointed to the possibility that an infectious agent could be involved. A recent study published in Science magazine used unbiased metagenomic analysis to survey microflora present in normal and CCD-affected hives to determine whether a pathological agent could be linked to CCD. The authors found that the presence of one virus, Israeli acute paralysis virus of bees (IAPV), showed a strong correlation with colony collapse disorder. In addition to the important economical implications, this work also represents a novel use for massively parallel next generation sequencing technology which has enabled this type of high level metagenomic study.

You will hear our panel, which includes two of the study's authors, discussing:
- how metagenomics can be applied in the discovery of unknown pathogens
- the importance of study design and data analysis in metagenomics research
- how recent technological advances have made this type of study possible

Participants:
W. Ian Lipkin, M.D.
Professor of Epidemiology and
Professor of Neurology and Pathology
Mailman School of Public Health
Columbia University
New York , NY

Michael Egholm, Ph.D.
Vice President of Research & Development
454 Life Sciences
Branford , CT

REGISTER NOW AT: www.sciencemag.org/webinar

Wednesday, 10 October 2007

Buy EVOcard to Receive Christmas presents

 
 

evocard

Buy your EVOcard and receive

2 Christmas presents

on orders placed until 24th December for oligos & sequencing

 

Up to 35% discount off the list price on your MWG orders
(35% discount on unmodified oligonucleotides; 15% discount
on sequencing reactions and modified oligonucleotides)

Amazon vouchers or EVOcard bonus
(7.5% of total order spend until 24/12/07 arwarded as Amazon
vouchers; 15% of total order spend from until 24/12/07 applied
to account as EVOcard bonus)

Please register by email to be eligible for the bonus - send
contact details and your preference of EVOcard bonus or
Amazon vouchers to evocardpromotion@mwgdna.com or
faxback this postcard to + 49 80928289970.

 

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Millipore Cell Signaling Application Guide

Invaluable, up-to-date information on one of the most critical life science workflows—Cell Signaling—is now available.
Are you looking for the latest products and technical information in cell signaling? With more than 500 pages of products, workflows and technical reference material the New Cell Signaling Application Guide provides the most comprehensive support and advanced selection of tools available in the area of cell signaling.


Cell Signaling Application and Product Guide   The Cell Signaling Application Guide includes dozens of cell signaling pathways, over 100 pages of technical articles, an extensive troubleshooting section, as well as thousands of Upstate cell signaling products including detailed product information in the following areas:


• Upstate enzymes, antibodies and kits
• Epigenetics and Chromatin Function
• Multiplexing and Drug Discovery Services
• Pathway Diagrams


Request your copy of the Cell Signaling Application Guide.

Real-time PCR Video for GM soybean DNA

PCR Animation

PCR Tutorial Animation

Molecular Structure Animation

Interleukin-1 binding to its receptor on a cell surface, created from structural data

SDS-PAGE VIDEO

Biochemical Applications 101 - Sodium dodecylsuplhate gelelectrophoresis (SDS-PAGE)

Video SDS PAGE

A video showing SDS polyacrylamide gel electrophoresis in ~120x time (constant voltage). Note the stained markers on the right.

Gel Electrophoresis and Blotting 2

Gel Electrophoresis and Blotting video

James giving a talk at Hunter College on Gel Electrophoresis and Blotting.

Tuesday, 9 October 2007

Receive 50% off new GPCR division arrested cells

Receive 50% off new GPCR division arrested cells
Invitrogen's Division Arrest (DA) technology for GPCRs allows you to use frozen cells as ordinary, cost-effective assay reagents for screening. Now for a limited time you can choose from more than 40 of our GeneBLAzer® GPCR division arrested cells at 50% off the list price - simply quote P392640 when you place your order.
Sample data for selected targets can be obtained by clicking in the table below.

H1
H1-NFAT-bla HEK293T
K1299
H1.pdf

H2
H2-CRE-bla HEK293T
K1307
H2.pdf

EDG3
EDG3-Ga15-NFAT-bla HEK293T
K1319
EDG3.pdf

EDG7
EDG7-NFAT-bla HEK293T
K1315
EDG7.pdf

D2
D2-Gqo5-NFAT-bla CHO-k1
K1309
D2-Gqo5.pdf

View the complete list of GeneBLAzer® Division Arrested GPCR cells.

Monday, 8 October 2007

Stem Cell Primer

The Politics of Stem Cell Research

Get Developmental Cell for $99!

SPECIAL OFFER:
Get Developmental Cell for $99!

Your 1-year personal print subscription includes:

• 12 monthly issues, filled with fundamental advances in the fields of cell and developmental biology;
• Reviews, commentaries and analyses complementing the issue's primary research papers; and
• Free, full-text online access to Developmental Cell (including early publication material and the online archive).

Subscribe for $99 today! (When prompted for a Discount Code, enter i00307.)

Friday, 5 October 2007

New Qubit® fluorometer from Invitrogen

Are DNA/RNA quantitation, time, and money related? With accurate
quantitation, you decide how much DNA/RNA to use in experiments—
giving more reliable results. The Qubit® fluorometer is more
accurate than UV absorbance, saving time and money. Try our demo at
www.invitrogen.com/qubitsavesyoumoney.

Science Podcast

Science Podcast
Science PodcastJoin host Robert Frederick for interviews and stories on attaching cameras to birds to study their behavior; a downside to breeding fish in captivity; making your lab and scientific conference environmentally friendly; and more. It's all free. Go to:
http://www.sciencemag.org/multimedia/podcast/#20071005.

Thursday, 4 October 2007

NEW! Cell's Latest Podcast

NEW! Cell's Latest Podcast

In our newest podcast, Dr. Emilie Marcus talks to Dr. Rudi Grosschedl about how gene regulation can be coordinated by interactions between chromosomes, and we hear from Dr. Jim Collins about a new way in which common antibiotics can kill bacteria. We also learn about some of the exciting research published in Cell in the last few months, including a study that shows how bones can keep you slim and give you energy. Listen to Cell's Podcast.

Western analysis in one day

Tough protein separation?
Get more novel, more Novex(r). Do a Western analysis in one day,
efficiently analyze native proteins, transfer proteins in 7 minutes.
Novex(r) products bring it all together. Isn't time your protein
analysis was as novel as your thinking? Get novel at
www.invitrogen.com/NOVEX.

SilenciX cell lines-Already silenced stable cell lines

SilenciX cell lines
Unique in Europe: Already silenced stable cell lines
Our pre-silenced SilenciX cell lines is a breakthrough in knock-down expression. Such
catalog product is only available at tebu-bio.
Don't wait anymore to benefit from our first stable silenced cell system:
*   Extinction already performed: from shRNA design to validation by qPCR, what
    you just need to do is to thaw the cells!
*   Over 70% knockdown guaranteed
*   Long-term stability > 500 days
*   Control included: you receive 1 vial of "target specific" SilenciX cells validated
    by qPCR and 1 vial of control SilenciX cells (transfected with a plasmid encoding
    for a non-relevant sequence).
*   No virus infection: No particular handling safety is needed
*   For 4,800 € only (£ 3,265)
    Your target does not appear in our current list? We will put it at the top of our
    production plan.
Customization for cell types (human) and targets is also possible. For further
information, you can contact our product specialist  AurĂ©lie SERRA.
www.tebu-bio.com: the smarter way to buy research products

Wednesday, 3 October 2007

ClonePix FL

ClonePix FL: powerful technology to explore cell surfaces and discover stellar clones. Select and isolate mammalian clones by cell surface protein expression, rapidly yielding monoclonal populations for cell based assays, screening and discovery research. Significantly improve timelines, labor costs and efficiency of cell line selection.

Nuclear Receptors in Liver and Digestive Diseases: A Research Workshop

Sponsored by: Division of Diabetes, Endocrinology, and Metabolic Diseases (DEM) and
Division of Digestive Diseases and Nutrition (DDN)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)

Description of the Meeting:

The nuclear receptor (NR) superfamily of transcriptional regulators has been integrally involved in the regulation of a variety of genes related to such diverse cellular and systems processes as the control of development to the regulation of bile acid and cholesterol biosynthesis. Disruption of the NR signaling pathways has been implicated in a variety of disease states such as diabetes, obesity, liver diseases such as non-alcoholic steatohepatitis, and other endocrine-related disorders. Many of the NR-implicated liver diseases are areas of high priority for research as outlined in the Trans-NIH Action Plan for Liver Disease Research (http://www.niddk.nih.gov/fund/divisions/ddn/ldrb/ldrb_action_plan.htm). Additionally, the NIDDK has recognized the importance of NRs in a variety of disease states and currently supports the Nuclear Receptor Signaling Atlas (http://www.nursa.org/index.cfm), an open access web portal for information about the NR superfamily.
This meeting will bring together scientists focused on the mechanisms and basic science of NR transcriptional regulation and translational and clinical investigators focused on the pathophysiology of digestive and liver disease states where an NR pathway has been implicated. Two keynote talks will cover the current basic understanding of NR regulation and the potential role of NR signaling dysregulation in the pathophysiology of disease conditions. Other talks will explore the known physiologic roles of ligands and receptors, such as sterol regulatory element-binding protein (SREBP), peroxisome proliferator-activated receptor (PPAR), retinoid X receptor (RXR), liver X receptor (LXR), and small heterodimer partner (SHP), and their downstream signaling in normal and in specific clinical conditions. The signaling mechanisms implicated in the development of obesity, non-alcoholic steatohepatitis, diabetes, bile acid and cholesterol synthesis dysregulation, and so on, also will be explored.
The aim of this meeting is to review the present state-of-the art knowledge of NRs, to promote cross-fertilization among the spectrum of basic, translational, and clinical investigators, and to integrate the current understanding of NR biology and the current clinical challenges for a variety of digestive and liver disease states. At the conclusion of the meeting, the organizers will define research challenges identified during the meeting. A meeting summary manuscript, including the research challenges identified, will be submitted for publication.

Organizers:

Edward Doo, Liver Disease Research Branch (LDRB), DDN, NIDDK
Ronald Evans, The Salk Institute for Biological Studies
Saul Karpen, Baylor College of Medicine
Joel Lavine, University of California at San Diego
Ronald Margolis, DEM, NIDDK
Patricia Robuck, LDRB, DDN, NIDDK

Locked Nucleic Acid-based arrays

The next generation of Exiqon's popular Locked Nucleic Acid-based arrays - the miRCURYTM LNA microRNA Array - is now available. With unmatched specificity, you can conduct expression profiling of a comprehensive range of microRNAs from any organism you like - vertebrates, invertebrates, plants and viruses, with unmatched sensitivity (sample from just 30 ng total RNA). You even have access to 150 proprietary microRNAs unavailable elsewhere (miRPlusTM). Our microRNA array platform is part of Exiqon's miRCURYTM product line, the most complete range of tools for microRNA analysis available today. The miRCURYTM tools enable you to seek, find and verify microRNAs - and to accelerate your discoveries.
www.exiqon.com/array-embo

Invitrogen Cellular Analysis 2007

The 2007 catalog from Invitrogen Cellular Analysis presents a broad portfolio of enabling inventions and their applications, brought together specifically to meet the challenges of examining biology in context. Order your copy today at www.invitrogen.com/cellsrock

Neuroscience Recruitment Advertising Package

Are you recruiting for researchers, post docs, faculty or staff in the field of neuroscience?

Satisfy your recruitment needs with our Neuroscience Recruitment Advertising Package!
Your advertisement will be featured in print in New Scientist magazine, the Neuron Special Supplement on Neuroscience Jobs & Trends, and online on ScienceJobs.com! Contact us at nssales@elsevier.com to learn about our discounted package rates and bonus distribution to the SfN Annual Meeting.

Transfection Research Award

Celebrate 10 Years of FuGENE® Reagents in Washington, D.C. in December!
Don't miss the chance to apply for the Transfection Research Award, and be eligible to win a trip to present your FuGENE® data at the 2007 ASCB Annual Meeting in Washington, D.C.!
Visit 10th Anniversary website for details and more information.

Additional GPCR Expressing Cell Lines Released

Lonza Releases Four New GPCR Expressing Cell Lines

 

Now available!  New cell lines for GPR103 and Vasopressin receptors V1a, V1b, and V2 allow you to evaluate excellent GPCR response levels at competitive prices. New receptors are available for the following therapeutic areas: increased feeding, blood pressure, bone formation, cell proliferation, glycogenolysis, and antidiuretic action.  Please review these GPCRs or our growing list of other GPCR products by going to our e-catalog. Place an order for any of the 4 products listed in this e-mail by October 30, 2007 and reference the code GPCRT207 to receive a 15% discount from list price.

>> Human GPR103 Cell Line

GPR103 is a G-protein coupled receptor that has been shown to be expressed primarily in brain, heart, kidney, adrenal gland, retina and testis. Its ligand has been identified as a 43-residue RF-amide peptide known as QRFP.

>> Human Vasopressin V1a Receptor Cell Line

The pituitary hormone vasopressin (AVP) is a cyclic nonapeptide that acts by binding to a family of vasopressin receptors that includes the V1a, V1b, and V2 receptors. V1a receptors have been shown to be expressed in vascular smooth muscle, hepatocytes, various blood cells, brain, and retina.

>> Human Vasopressin V1b Receptor Cell Line

The pituitary hormone vasopressin (AVP) is a cyclic nonapeptide that acts by binding to a family of vasopressin receptors that includes the V1a, V1b, and V2 receptors. V1b receptors are expressed in the anterior pituitary where they mediate ACTH release.

>> Human Vasopressin V2 Receptor Cell Line

V2 receptors are expressed in the kidney where vasopressin exerts its antidiuretic action.

Science Protocols