Receive 50% off new GPCR division arrested cells

Receive 50% off new GPCR division arrested cells
Invitrogen's Division Arrest (DA) technology for GPCRs allows you to use frozen cells as ordinary, cost-effective assay reagents for screening. Now for a limited time you can choose from more than 40 of our GeneBLAzer^® GPCR division arrested cells at 50% off the list price - simply quote P392640 when you place your order.
Sample data for selected targets can be obtained by clicking in the table below.

H1
H1-NFAT-bla HEK293T
K1299
H1.pdf

H2
H2-CRE-bla HEK293T
K1307
H2.pdf

EDG3
EDG3-Ga15-NFAT-bla HEK293T
K1319
EDG3.pdf

EDG7
EDG7-NFAT-bla HEK293T
K1315
EDG7.pdf

D2
D2-Gqo5-NFAT-bla CHO-k1
K1309
D2-Gqo5.pdf

View the complete list of GeneBLAzer^® Division Arrested GPCR cells.

ClonePix FL

ClonePix FL: powerful technology to explore cell surfaces and discover stellar clones. Select and isolate mammalian clones by cell surface protein expression, rapidly yielding monoclonal populations for cell based assays, screening and discovery research. Significantly improve timelines, labor costs and efficiency of cell line selection.

New Mycoplasma Elimination Reagent Lonza

When cell lines are too precious to discard...

MycoZap™ Mycoplasma Elimination Reagent may be the answer.

Reports are that 5-35% of all cells in continuous culture are contaminated with mycoplasma. A cell line infection can affect every known process in the cell and can seriously impact the reliability, reproducibility and consistency of results. Additionally, mycoplasma infections can spread easily within the culture environment. 

To monitor for such infections, regular testing of cell lines should be performed using Lonza’s MycoAlert^® Mycoplasma Detection Kit. Where contamination has occurred, and the sample absolutely cannot be discarded, the MycoZap™ Mycoplasma Elimination Reagent has been optimized to eliminate mycoplasma with minimal toxic effects on the cells.

MycoZap™ Mycoplasma Elimination Reagent is:

Easy – 
simply add the reagent to your culture media

Universal –
MycoZap™ can be used to eradicate Mollicutes, including Mycoplasma, Acholeplasma, Spiroplasma and Entomoplasma species in cell cultures

Thorough –
the combination of antibiotic and antimetabolic agents ensure total removal of the contamination

Complete –
the kit contains all the required reagents

Click here to learn more about MycoZap™ and how you can
      save 10% on MycoAlert® Mycoplasma Detection kits.

Protocols for Neural Cell Culture

* Publisher: Humana Press
* Number Of Pages: 384
* Publication Date: 2001-02-15
* Sales Rank: 1339161
* ISBN / ASIN: 0896039021
* EAN: 9780896039025
* Binding: Plastic Comb
* Manufacturer: Humana Press
* Studio: Humana Press

Sergey Fedoroff and Arleen Richardson extensively revise, update, and expand their best-selling and highly praised collection of readily reproducible neural tissue culture protocols. This 3rd edition adds 11 new chapters describing important new procedures for the isolation, growth, and characterization of neural stem cells and for the manipulation of glial progenitor cells, as well as are essential procedures for hippocampal and microglial slice cultures. Protocols for Neural Cell Culture: Third Edition is a richly augmented updating of the tried and tested laboratory procedures that have made earlier editions an indispensable reference and guide to neural cell culture and its disorders.

Link

Protocols Ebook for Cryopreservation and Freeze-Drying (Methods in Molecular Biology)

• Publisher: Humana Press
• Number Of Pages: 254
• Publication Date: 1995-03-24
• Sales Rank: 2247135
• ISBN / ASIN: 0896032965
• EAN: 9780896032965
• Binding: Paperback
• Manufacturer: Humana Press
• Studio: Humana Press
• 
  Book Description:

  This book provides detailed protocols for all the latest methodologies used to assure the long-term biostorage of a diverse range of biological materials.
  Developed in expert laboratories, the protocols have been painstakingly perfected over the years to provide time-tested, step-by-step instructions that ensure robust and reproducible results. Each protocol deals with the preservation of an organelle, cell, or tissue type, and is accessible even to the nonspecialist because of its cookbook approach. Novel protocols can be readily developed with the help of the "hands-on" Notes sections.
  Cryopreservation and Freeze-Drying Protocols is an indispensable reference work for both the individual researcher, and all those who want to establish or improve biostorage systems in their laboratories. Its applications range from microbial culture collections, botanic gardens, and zoos to animal husbandry, aquaculture, medicine, human fertilization, and cell and molecular biology.

Link

How to do Mixed-cell-culture assays for analyzing neuronal synapse formation

The assembly of synapses in the vertebrate central nervous system requires bidirectional signaling across the synaptic cleft that directs the differentiation of pre- and postsynaptic membrane domains. Biochemical and genetic studies have identified several adhesion and signaling molecules that localize to synapses and might participate in organizing synaptic structures. Understanding how individual proteins contribute to synaptic organization is complicated by the fact that there are significant numbers of separate signals that cooperate in this process. This protocol describes an assay system that permits examination of synaptogenic activities of individual cell-surface proteins in isolation. Besides the time needed for preparation and growth of primary neuronal cultures (6-14 days), the execution and analysis of the assay is rapid, requiring approximately 2 days. Using this assay, recent studies revealed that single synaptic adhesion complexes can direct a remarkable degree of synaptic differentiation and provided new insights into the cell biological mechanisms of synaptogenesis.

Full text

How to Prepare a copper-based fluorescent probe for nitric oxide and use it in mammalian cultured cells

 

A procedure for the preparation of a copper(II) complex (CuFL) as a fluorescent nitric oxide (NO) detector is described. The fluorescein-based ligand FL can be synthesized in seven reaction steps (overall yield ?20%), typically requiring a total time of 9 days. The CuFL probe allows for the detection of NO produced in mammalian cultured cells. The detailed protocol for the use of CuFL for imaging NO in human neuroblastoma SK-N-SH cells takes a total time of ?26 h. This includes plating cells on six-well tissue culture plates or imaging dishes, treatment with CuFL, stimulation of NO synthases and imaging by fluorescence microscopy.

Full Text

Freezing and Thawing Cultured Cells

 

From Allan Bradley's Lab, Baylor College of Medicine, Houston

View

Freezing and Thawing Eukaryotic Cells

From Dr. Bart Frank Library, Arthritis and Immunology Program, OMRF

View

Freezing and Thawing of Mammalian Cell Lines

 

From The University of Texas at Austin

View

Freezing cells in liquid nitrogen

For T25 Flask

1. Take off supernatant
2. Trypsinization with 1ml 0.25% EDTA
3. Give a hard Shock to the flask to remove all attached cells
4. Add 10ml Media +10%FCS
5. Pipette up and down to distribute cells throughout media (i.e. not clumped together)
6. Add resuspended cells to sterile universal tube 
7. Spin down 1500rpm, 3 mins
8. Take off media
9. Resuspend pellet in 2ml FCS +10%DMSO
10. Distribute in 500ul aliquots (0.5 to 2 Million cells/ml)
11. Move cells to -80^oC overnight in Mr. frosty box (filled with ethenol)
12. Finally freeze cells in liquid N₂

Unthawing

1. Warm DMEM in waterbath
2. Thaw cryovial at 37^oC quickly until cells become molten.

Aliquot the 1ml of cells using a disposable pipette into 10ml fresh media in a TC flask

Cell Thawing/Freezing Protocol

From University of Chicago

View

Cryopreservation Manual (Nalge Nunc)

 

Download

Cryogenic Preservation and Storage of Animal Cells

 

Download

Tissue Culture - Storage of Cell Lines

From Hancock Laboratory Methods

Download