Cytokines and growth factors array

CYTOKINES & GROWTH FACTORS RESEARCH BIOCHIPS

Randox supplies biochips for all your cytokines & growth factors research. Biochips offer multiplexing; all analytes are measured simultaneously from one sample. Biochip array technology brings analytical clinical standards to research. Randox biochips are ready-to-use on the evidence investigator™ complete with controls and calibrators.

Cytokines and growth factors are proteins secreted primarily by immune system cells such as leucocytes. They are a means of intercellular communication with both immune and non-immune system cells. Upon binding to a cell membrane receptor, cytokines initiate a signal transduction cascade in their target cell. Cytokines form a complex web of interactions since:

Different cytokines can have similar effects.

Cells interact with a variety of cytokines.

Cytokines may affect the activity and expression of other cytokines.

evidence investigator
The evidence investigator is a compact bench-top Biochip Array Technology analyser for the simultaneous analysis of a panel of tests in a single sample. The Clinical standards for research page gives an overview of Biochip Array Technology.
The cytokines and growth factors array of tests are presented on a single biochip. All twelve tests are performed simultaneously using just one assay diluent, one panel-specific conjugate solution and one signal reagent.
Cytokines and growth factors array test menu
Interleukin-1 alpha (IL-1a)
Interleukin-1 beta (IL-1b)
Interleukin-2 (IL-2)
Interleukin-4 (IL-4)
Interleukin-6 (IL-6)
Interleukin-8 (IL-8)
Interleukin-10 (IL-10)
Interleukin-12 (IL-12) - in development
Vascular Endothelial Growth Factor (VEGF)
Tumour Necrosis Factor-alpha (TNF-a)
Interferon-gamma (IFN-g)
Epidermal Growth Factor (EGF)
Monocyte Chemotactic Protein-1 (MCP-1)
For more detailed information please e-mail: marketing@randox.com

Details: CYTOKINES & GROWTH FACTORS RESEARCH BIOCHIPS

Basic Immunology Ebook: Functions and Disorders of the Immune System Code- by Abul K. Abbas, Andrew H. Lichtman

The 2nd of this popular text emphasizes the fundamental concepts and principles of human immunology that students need to know, without overwhelming them with extraneous material. It leads the reader to a firm understanding of basic principles, using full-color illustrations; short, easy-to-read chapters; color tables that summarize key information clinical cases; and much more-all in a conveniently sized volume that's easy to carry. The New Edition has been thoroughly updated to reflect the many advances that are expanding our understanding of the field.

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Encyclopedia of Immunology-Ebook, Second Edition

With more than 700 expert authors from 22 different countries, the Encyclopedia of Immunology, Second Edition is the largest comprehensive reference source of current immunological knowledge available. It provides a broad scope and high level of expertise to the many aspects of the field of immunology and related areas, including microbiology, virology, and parasitology. Arranged into 31 subject areas with extensive cross-referencing and subject indexes in each volume, the Encyclopedia is easy-to-use for virtually any researcher, regardless of his or her field. Concise definitions of the subject area also introduce each entry. The Second Edition includes timely and thorough updates for all articles from the First Edition, more than 60 new entries, a glossary of immunological terms in each volume, a total of 500 figures and tables, and new color plates sections.

Key Features
* Four volumes, each containing a subject index
* Approximately 630 different articles
* More than 700 expert contributors from 22 different countries
* Coverage of 31 different subject areas
* Concise definitions of the subject to introduce each entry
* Further reading lists at the end of each entry
* Extensive cross-referencing
* Entries arranged in a single A-Z list for easy access
* Easy-to-read double-column format
* More than 500 figures to complement the text
* More than 60 new articles
* A glossary of immunological terms in each volume
* A color plate section in each volume

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Immunology Guidebook-E Book

The Immunology Guidebook provides an easily accessible text-reference to the more
up-to-date and difficult concepts in the complex science of immunology. It aims to demystify basic concepts and specialised molecular and cellular interactions. Its 18 chapters offer a logical and sequential presentation where much of the data is displayed in carefully designed tables. This book is intended for immunology students, researchers, practitioners and basic biomedical scientists.
* Tables provide a quick reference to difficult to find immunology data
* A distillate of the latest information on immunogenetics of the human MHC associated with tissue transplantation
* Information boxes featurw related web resources

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Statistical Methods in Bioinformatics: An Introduction-E-Book

• Hardcover: 588 pages
• Publisher: Springer; 2 edition (September 30, 2005)
• Language: English
• ISBN-10: 0387400826

Advances in computers and biotechnology have had a profound impact on biomedical research, and as a result complex data sets can now be generated to address extremely complex biological questions. Correspondingly, advances in the statistical methods necessary to analyze such data are following closely behind the advances in data generation methods. The statistical methods required by bioinformatics present many new and difficult problems for the research community.

This book provides an introduction to some of these new methods. The main biological topics treated include sequence analysis, BLAST, microarray analysis, gene finding, and the analysis of evolutionary processes. The main statistical techniques covered include hypothesis testing and estimation, Poisson processes, Markov models and Hidden Markov models, and multiple testing methods.

The second edition features new chapters on microarray analysis and on statistical inference, including a discussion of ANOVA, and discussions of the statistical theory of motifs and methods based on the hypergeometric distribution. Much material has been clarified and reorganized.

The book is written so as to appeal to biologists and computer scientists who wish to know more about the statistical methods of the field, as well as to trained statisticians who wish to become involved with bioinformatics. The earlier chapters introduce the concepts of probability and statistics at an elementary level, but with an emphasis on material relevant to later chapters and often not covered in standard introductory texts. Later chapters should be immediately accessible to the trained statistician. Sufficient mathematical background consists of introductory courses in calculus and linear algebra. The basic biological concepts that are used are explained, or can be understood from the context, and standard mathematical concepts are summarized in an Appendix. Problems are provided at the end of each chapter allowing the reader to develop aspects of the theory outlined in the main text.

Warren J. Ewens holds the Christopher H. Brown Distinguished Professorship at the University of Pennsylvania. He is the author of two books, Population Genetics and Mathematical Population Genetics. He is a senior editor of Annals of Human Genetics and has served on the editorial boards of Theoretical Population Biology, GENETICS, Proceedings of the Royal Society B and SIAM Journal in Mathematical Biology. He is a fellow of the Royal Society and the Australian Academy of Science.

Gregory R. Grant is a senior bioinformatics researcher in the University of Pennsylvania Computational Biology and Informatics Laboratory. He obtained his Ph.D. in number theory from the University of Maryland in 1995 and his Masters in Computer Science from the University of Pennsylvania in 1999.

Comments on the First Edition. "This book would be an ideal text for a postgraduate course.[and] is equally well suited to individual study.. I would recommend the book highly" (Biometrics). "Ewens and Grant have given us a very welcome introduction to what is behind those pretty [graphical user] interfaces" (Naturwissenschaften.). "The authors do an excellent job of presenting the essence of the material without getting bogged down in mathematical details" (Journal. American Staistical. Association). "The authors have restructured classical material to a great extent and the new organization of the different topics is one of the outstanding services of the book" (Metrika).

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Cancer Medicine- Ebook, Kufe, Donald W.; Pollock, Raphael E.; Weichselbaum, Ralph R.; Bast, Robert C., Jr.; Gansler, Ted S.; Holland

Kufe, Donald W.; Pollock, Raphael E.; Weichselbaum, Ralph R.; Bast, Robert C., Jr.; Gansler, Ted S.; Holland, James F.; Frei III, Emil, editors. Hamilton (Canada): BC Decker Inc. ; c2003

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Natural Killer Cell Protocols: Cellular and Molecular Methods

By Kerry S. Campbell, M
.D. M Colonna

Contributor Kerry S
. Campbell, Marco
Colonna

Published 2000
Humana Press

Medical / Nursing

500 pages, ISBN 0896036839

Natural Killer Cell Protocols: Cellular and Molecular Methods offers immunologists, cancer researchers, virologists, and cell biologists today's most comprehensive collection of both established and cutting-edge techniques, methods that will contribute significantly to advancing our understanding of this fascinating and critically important class of cells.

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Immunochemical Protocols By Johan D. Pound- E-book

By Johan D. Pound

Published 1998
Humana Press

Science

528 pages

ISBN 0896033880

 

This much anticipated second edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for bioscientists. It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins and their various conjugates. This book will open the door to a new generation of immunochemical reagents with exciting possibilities.

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Freezing and Thawing Cultured Cells

 

From Allan Bradley's Lab, Baylor College of Medicine, Houston

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Freezing and Thawing Eukaryotic Cells

From Dr. Bart Frank Library, Arthritis and Immunology Program, OMRF

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Freezing and Thawing of Mammalian Cell Lines

 

From The University of Texas at Austin

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Freezing cells in liquid nitrogen

For T25 Flask

1. Take off supernatant
2. Trypsinization with 1ml 0.25% EDTA
3. Give a hard Shock to the flask to remove all attached cells
4. Add 10ml Media +10%FCS
5. Pipette up and down to distribute cells throughout media (i.e. not clumped together)
6. Add resuspended cells to sterile universal tube 
7. Spin down 1500rpm, 3 mins
8. Take off media
9. Resuspend pellet in 2ml FCS +10%DMSO
10. Distribute in 500ul aliquots (0.5 to 2 Million cells/ml)
11. Move cells to -80^oC overnight in Mr. frosty box (filled with ethenol)
12. Finally freeze cells in liquid N₂

Unthawing

1. Warm DMEM in waterbath
2. Thaw cryovial at 37^oC quickly until cells become molten.

Aliquot the 1ml of cells using a disposable pipette into 10ml fresh media in a TC flask

Cell Thawing/Freezing Protocol

From University of Chicago

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Cryopreservation Manual (Nalge Nunc)

 

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Cryogenic Preservation and Storage of Animal Cells

 

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Tissue Culture - Storage of Cell Lines

From Hancock Laboratory Methods

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THe Immune System

Cytokine Protocols (Methods in Molecular Biology)

Series: Methods in Molecular Biology
Pub Date: October 2003
Pages: 248
ISBN: 0-89603-948-X
DOI: 10.1226/089603948X

Table of Contents

Large-Scale Generation of Plasmids that Express Type I Interferon
Peter Härle and Daniel J. J. Carr

Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay
Rebecca J. Chan, Min You, and Gen-Sheng Feng

Modulation of the Interferon-g Signal by Transfection of Cells with an
Antisense-RNA Expressing Vector
Leen Delrue and Marc De Ley

Competitive RT-PCR to Quantify Small Amounts of mRNA
Gabriela Aust

In Situ Hybridization for Cytokines in Human Tissue Biopsies
Emily Mathey, John Pollard, and Patricia Armati

Purification, Identification, and Synthesis of Chemokines
Patricia Menten, Anja Wuyts, and Jo Van Damme

Receptor Isolation and Characterization: From Protein to Gene
Daniela Novick and Menachem Rubinstein

Crystallization of Cytokine–Receptor Complexes
William J. Cook and Mark R. Walter

Biosensor Analysis of Receptor–Ligand Interactions
Sheng-Jiun Wu and Irwin Chaiken

Analysis of SH2 Ligands and Identification of Sites of Interaction
Manuel Baca

Assays for Antiviral Activity
Anthony Meager

Assays for Cytotoxicity
Anthony Meager

Assays for Chemotaxis
Anja Wuyts, Patricia Menten, Nancy Van Osselaer, and Jo Van Damme

In Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of
Granulocyte Colony-Stimulating Factor
James F. Eliason, Nadine Tare, Sharon Bowen, Tomoaki Inoue, and Ikuro Horii

Development of a Mammalian Tet-On Expression Cell Line: Glucosylceramide
Synthase Regulates TNF-a-Induced Apoptosis
Yong-Yu Liu and Myles C. Cabot

Immunohistochemical Detection of Cytokines in Human Tissue Sections
Sandra Hulshof, Danny F. Dukers, Wim Vos, and Corline J. A. De Groot

Detection of Cytokine- and Chemokine-Expressing Cells at the
Single Cell Level
Karin Loré and Jan Andersson

Detection of Cytokine Signal Transduction “Cross-Talk” in Leukocyte
Activation
Howard R. Petty

Assay System for the Effect of Prostaglandin E2 in the Determination of
Polarized Cytokine Production
Etsushi Kuroda and Uki Yamashita

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