Series: Methods in Molecular Biology
Pub Date: October 2003
Pages: 248
ISBN: 0-89603-948-X
DOI: 10.1226/089603948X
Table of Contents
Large-Scale Generation of Plasmids that Express Type I Interferon
Peter Härle and Daniel J. J. Carr
Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay
Rebecca J. Chan, Min You, and Gen-Sheng Feng
Modulation of the Interferon-g Signal by Transfection of Cells with an
Antisense-RNA Expressing Vector
Leen Delrue and Marc De Ley
Competitive RT-PCR to Quantify Small Amounts of mRNA
Gabriela Aust
In Situ Hybridization for Cytokines in Human Tissue Biopsies
Emily Mathey, John Pollard, and Patricia Armati
Purification, Identification, and Synthesis of Chemokines
Patricia Menten, Anja Wuyts, and Jo Van Damme
Receptor Isolation and Characterization: From Protein to Gene
Daniela Novick and Menachem Rubinstein
Crystallization of Cytokine–Receptor Complexes
William J. Cook and Mark R. Walter
Biosensor Analysis of Receptor–Ligand Interactions
Sheng-Jiun Wu and Irwin Chaiken
Analysis of SH2 Ligands and Identification of Sites of Interaction
Manuel Baca
Assays for Antiviral Activity
Anthony Meager
Assays for Cytotoxicity
Anthony Meager
Assays for Chemotaxis
Anja Wuyts, Patricia Menten, Nancy Van Osselaer, and Jo Van Damme
In Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of
Granulocyte Colony-Stimulating Factor
James F. Eliason, Nadine Tare, Sharon Bowen, Tomoaki Inoue, and Ikuro Horii
Development of a Mammalian Tet-On Expression Cell Line: Glucosylceramide
Synthase Regulates TNF-a-Induced Apoptosis
Yong-Yu Liu and Myles C. Cabot
Immunohistochemical Detection of Cytokines in Human Tissue Sections
Sandra Hulshof, Danny F. Dukers, Wim Vos, and Corline J. A. De Groot
Detection of Cytokine- and Chemokine-Expressing Cells at the
Single Cell Level
Karin Loré and Jan Andersson
Detection of Cytokine Signal Transduction “Cross-Talk†in Leukocyte
Activation
Howard R. Petty
Assay System for the Effect of Prostaglandin E2 in the Determination of
Polarized Cytokine Production
Etsushi Kuroda and Uki Yamashita
Pub Date: October 2003
Pages: 248
ISBN: 0-89603-948-X
DOI: 10.1226/089603948X
Table of Contents
Large-Scale Generation of Plasmids that Express Type I Interferon
Peter Härle and Daniel J. J. Carr
Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay
Rebecca J. Chan, Min You, and Gen-Sheng Feng
Modulation of the Interferon-g Signal by Transfection of Cells with an
Antisense-RNA Expressing Vector
Leen Delrue and Marc De Ley
Competitive RT-PCR to Quantify Small Amounts of mRNA
Gabriela Aust
In Situ Hybridization for Cytokines in Human Tissue Biopsies
Emily Mathey, John Pollard, and Patricia Armati
Purification, Identification, and Synthesis of Chemokines
Patricia Menten, Anja Wuyts, and Jo Van Damme
Receptor Isolation and Characterization: From Protein to Gene
Daniela Novick and Menachem Rubinstein
Crystallization of Cytokine–Receptor Complexes
William J. Cook and Mark R. Walter
Biosensor Analysis of Receptor–Ligand Interactions
Sheng-Jiun Wu and Irwin Chaiken
Analysis of SH2 Ligands and Identification of Sites of Interaction
Manuel Baca
Assays for Antiviral Activity
Anthony Meager
Assays for Cytotoxicity
Anthony Meager
Assays for Chemotaxis
Anja Wuyts, Patricia Menten, Nancy Van Osselaer, and Jo Van Damme
In Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of
Granulocyte Colony-Stimulating Factor
James F. Eliason, Nadine Tare, Sharon Bowen, Tomoaki Inoue, and Ikuro Horii
Development of a Mammalian Tet-On Expression Cell Line: Glucosylceramide
Synthase Regulates TNF-a-Induced Apoptosis
Yong-Yu Liu and Myles C. Cabot
Immunohistochemical Detection of Cytokines in Human Tissue Sections
Sandra Hulshof, Danny F. Dukers, Wim Vos, and Corline J. A. De Groot
Detection of Cytokine- and Chemokine-Expressing Cells at the
Single Cell Level
Karin Loré and Jan Andersson
Detection of Cytokine Signal Transduction “Cross-Talk†in Leukocyte
Activation
Howard R. Petty
Assay System for the Effect of Prostaglandin E2 in the Determination of
Polarized Cytokine Production
Etsushi Kuroda and Uki Yamashita
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