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Saturday 26 May 2007

Cytokine Protocols (Methods in Molecular Biology)

Series: Methods in Molecular Biology
Pub Date: October 2003
Pages: 248
ISBN: 0-89603-948-X
DOI: 10.1226/089603948X

Table of Contents

Large-Scale Generation of Plasmids that Express Type I Interferon
Peter Härle and Daniel J. J. Carr

Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay
Rebecca J. Chan, Min You, and Gen-Sheng Feng

Modulation of the Interferon-g Signal by Transfection of Cells with an
Antisense-RNA Expressing Vector
Leen Delrue and Marc De Ley

Competitive RT-PCR to Quantify Small Amounts of mRNA
Gabriela Aust

In Situ Hybridization for Cytokines in Human Tissue Biopsies
Emily Mathey, John Pollard, and Patricia Armati

Purification, Identification, and Synthesis of Chemokines
Patricia Menten, Anja Wuyts, and Jo Van Damme

Receptor Isolation and Characterization: From Protein to Gene
Daniela Novick and Menachem Rubinstein

Crystallization of Cytokine–Receptor Complexes
William J. Cook and Mark R. Walter

Biosensor Analysis of Receptor–Ligand Interactions
Sheng-Jiun Wu and Irwin Chaiken

Analysis of SH2 Ligands and Identification of Sites of Interaction
Manuel Baca

Assays for Antiviral Activity
Anthony Meager

Assays for Cytotoxicity
Anthony Meager

Assays for Chemotaxis
Anja Wuyts, Patricia Menten, Nancy Van Osselaer, and Jo Van Damme

In Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of
Granulocyte Colony-Stimulating Factor
James F. Eliason, Nadine Tare, Sharon Bowen, Tomoaki Inoue, and Ikuro Horii

Development of a Mammalian Tet-On Expression Cell Line: Glucosylceramide
Synthase Regulates TNF-a-Induced Apoptosis
Yong-Yu Liu and Myles C. Cabot

Immunohistochemical Detection of Cytokines in Human Tissue Sections
Sandra Hulshof, Danny F. Dukers, Wim Vos, and Corline J. A. De Groot

Detection of Cytokine- and Chemokine-Expressing Cells at the
Single Cell Level
Karin Loré and Jan Andersson

Detection of Cytokine Signal Transduction “Cross-Talk” in Leukocyte
Activation
Howard R. Petty

Assay System for the Effect of Prostaglandin E2 in the Determination of
Polarized Cytokine Production
Etsushi Kuroda and Uki Yamashita

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