TE buffer (Tris-EDTA buffer) Recipe

The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.

10 mM Tris, bring to pH 8.0 with HCl, 1 mM EDTA

A stock can be prepared and stored at RT

Component For 500ml
1M Tris pH 8* 5ml
0.5M EDTA pH 8 1ml
dH2O 496ml

Autoclave to sterilize. It is then best to remove a working aliquot and not repeatedly access the stock.
*the pH is usually adjusted to RNA 8.0 for DNA and 7.5 for

How to make acetate-acetic acid buffer solutions

Sodium acetate-acetic acid buffer solutions, pH 3.7-5.6

Sodium acetate trihydrate, CH3COONa.3H2O, M.Wt. 136.09;
0.2M solution contains 27.22g/l.
Acetic acid, glacial is ~17.47M.

x ml 0.2M NaOAc and y ml 0.2M HOAc mixed.

pH, 18°C X mL

0.2M NaOAc

Y mL

0.2M HOAc
3.7 10.0 90.0
3.8 12.0 88.0
4.0 18.0 82.0
4.2 26.5 73.5
4.4 37.0 63.0
4.6 49.0 51.0
4.8 59.0 41.0
5.0 70.0 30.0
5.2 79.0 21.0
5.4 86.0 14.0
5.6 91.0 9.0