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Sunday, 9 November 2014

TE buffer (Tris-EDTA buffer) Recipe

The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.

10 mM Tris, bring to pH 8.0 with HCl, 1 mM EDTA

A stock can be prepared and stored at RT

Component For 500ml
1M Tris pH 8* 5ml
0.5M EDTA pH 8 1ml
dH2O 496ml

Autoclave to sterilize. It is then best to remove a working aliquot and not repeatedly access the stock.
*the pH is usually adjusted to RNA 8.0 for DNA and 7.5 for

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