Oxford Laboratory Technology: November 2007

Friday, 30 November 2007

Quick Blocking for Perfect Westerns

When the milk gets old, it's time to throw it away. Our 5M QuickBlock Kit can completely block any solid surface in just five minutes, reaching the same level of antigen detection with smaller amounts of antibody. http://www.biologyproject.net/quick_block_kit.html · Quick procedure: Efficiently blocks a western blot or ELISA plate in only five minutes. · Increased sensitivity: Significantly increases the ratio of signal over background noise, thus requires much less amount of antibody. · Wide range: Works with over 80% of the antibodies from the most popular species · Easy optimization: Determines the best blocking reagents for problematic antibodies · Low cost: The kits are designed only for blocking. There are no bundled reagents to inflate the price and depress your budget. Our customer representatives are available 24 hours, Monday through Friday. You may contact us anytime for assistance. Please check our contact page http://www.biologyproject.net/contact.html for our ocal reginal numbers.Western Blot Specialist Gen Script Corporation – VWR Strategic Partner 120 Centennial Ave., Piscataway, NJ 08854 Tel: 1-732-885-9188 ext 128 Fax: 1-732-210-0262

Custom Peptide Synthesis

Custom Peptide Synthesis

With almost 10 years experience in solid phase peptide synthesis, SBS Genetech is committed to supplying high quality peptides at competitive prices and providing our customers with the extensive services and products:• Purities from crude to 98% • Acetylation/Amidation • Phosphorylation/Fluorescein/Biotin labeled peptides • Cyclic peptides • KLH/BSA Conjugation • MAP peptides• 2-3 weeks turn-around time.

Check the price here
http://www.biocompare.com/optin/20071129_2077.html

Wednesday, 28 November 2007

Purification of miRNA from FFPE Sections

Purification of miRNA from FFPE Sections

The miRNeasy FFPE Kit from QIAGEN enables purification of total RNA that includes miRNA and other small RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections including laser capture microscopy samples. Fixing tissues with formalin leads to RNA–RNA and RNA–protein crosslinking, which impairs RNA performance in downstream applications. The miRNeasy FFPE Kit reverses formalin crosslinking and releases RNA from tissue sections while avoiding further RNA degradation. Optimized binding conditions allow purification of all usable RNA from approximately 18 nucleotides upwards. The purified RNA delivers maximal performance in a range of applications, including real-time PCR. To find out more about the miRNeasy FFPE Kit and QIAGEN's comprehensive range of miRNA research technologies, please visit the link below.

miRNeasy FFPE Kit

New online tool for LC method transfer

Better LC results, faster than ever.
Calculate it for yourself and compare
The Agilent 1200 Series Rapid Resolution LC (RRLC) system lets you keep
all your options open. With just one system you can push RRLC
performance to new limits and continue to run your conventional LC
methods, too.

Agilent's new online tool for LC method transfer
and cost calculation lets you make your own calculations and immediately
see the results:

Transfer your conventional LC methods
to RRLC methods
Compare equipment, consumables and
labor costs for conventional LC to RRLC
Try out the new Agilent LC method translator
plus cost calculator tool www.agilent.com/chem/RRLC_MT

Nanoparticle siRNA Transfection System

N-TER™ Nanoparticle siRNA Transfection System: siRNA Delivery to Difficult-to-Transfect Cells

Traditional lipid-based siRNA transfection reagents have a number of drawbacks, including a limited ability to transfect into a variety of cell types, such as primary, neuronal, differentiated, and non-dividing cells. Sigma's N-TER™ Nanoparticle siRNA Transfection System is a peptide based transfection reagent specifically designed to bypass these limitations and allow for efficient delivery of siRNAs into historically unapproachable eukaryotic cell lines. For more information, please visit us online at the link below.
more...

Novel Luciferase Assay System for HTS Applications

Targeting Systems Offers a Novel Luciferase Assay System for HTS Applications
This product is based on gaussia luciferase, a secreted luciferase that is more than a 1000 times brighter than firefly or renilla luciferases. When it comes to screening compounds that affect the activity of a weak promoter, an assay type and readout method can make the difference between actually finding compounds or proteins of interest and not finding them. Gaussia luciferase is extremely attractive for drug screening involving weak promoters. Ease of use, elimination of cell lysis, signal stability, and high signal intensity make the Secreted Gaussia Luciferase System a powerful tool for high-throughput applications. Targeting Systems also offers a dual luciferase reporter system based on gaussia luciferase with firefly luciferase as the second reporter.
more...

Tuesday, 27 November 2007

Reproducibility in Protein Biomarker Research WEBINAR

Please join our panel of experts on 5 December 2007 for a live online
educational seminar, "Tackling Reproducibility Issues in Mass
Spectrometry-based Biomarker Discovery."

Submit your questions LIVE to the experts during the webinar!

Date: Wednesday, December 5, 2007
Time: 12:00 noon EST; 9:00 am PST; 5 pm GMT
Duration: 1 hour

www.sciencemag.org/webinar

Mass spectrometry is an extremely powerful and sensitive technology that
can detect very small changes in expression levels. Some of these
changes may stem from the biological differences related to a disease or
treatment of interest. Others, however, may reflect the heterogeneity of
patients across multiple sites, the inherent biological complexity and
diversity of different sample types, and even small differences in
sample collection, processing, handling, and analysis techniques used by
multiple operators across multiple locations. As a consequence, data may
be tainted by site-, study-, population-, or sample-specific anomalies
and, therefore, not be sufficiently robust for biomarker discovery.

Join our panel of experts in a live, audience-driven Q&A as they discuss
how to overcome these reproducibility issues to generate less biased and
more reliable results. Questions for the panel can be submitted live
through your viewing console.

Participants:

Martin Latterich, Ph.D.
Faculty of Pharmacy
University of Montreal
Montreal, Canada

Timothy D. Veenstra, Ph.D.
SAIC-Frederick, Inc.
National Cancer Institute at Frederick
Frederick , MD

Toni Whistler, Ph.D.
Chronic Viral Diseases Branch
Centers for Disease Control and Prevention
Atlanta, GA
REGISTER NOW: www.sciencemag.org/webinar

--- Produced by the Science /AAAS Business Office and sponsored by
Bio-Rad ---

2007-2008 Sigma Antibodies Catalog

Introducing the 2007-2008 Sigma Antibodies Catalog
This new 320-page catalog includes:
* Over 4,000 high-quality antibodies
* Monoclonals, polyclonals, secondaries & conjugates
* Over 750 new antibodies
* Application photos
* Associated gene symbols
* Technical appendices
Click here to request your free copy online at
http://www.sigma.com/stkeabcatalog

BULL'S-EYE-InvitrogenT GPCR research tools

LESS MOVING TARGET, MORE BULL'S-EYE-Invitrogen(tm) GPCR research tools

Gain a clear advantage when interrogating difficult receptors with
integrated GPCR cellular assays from Invitrogen. The expert tools needed
to see the most lucrative targets, including target assays with multiple
readout options, are right in front of you. Learn more and sign up for
the Drug Discovery E-newsletter at www.invitrogen.com/gpcr.