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Wednesday, 28 November 2007

Purification of miRNA from FFPE Sections

Purification of miRNA from FFPE Sections

The miRNeasy FFPE Kit from QIAGEN enables purification of total RNA that includes miRNA and other small RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections including laser capture microscopy samples. Fixing tissues with formalin leads to RNA–RNA and RNA–protein crosslinking, which impairs RNA performance in downstream applications. The miRNeasy FFPE Kit reverses formalin crosslinking and releases RNA from tissue sections while avoiding further RNA degradation. Optimized binding conditions allow purification of all usable RNA from approximately 18 nucleotides upwards. The purified RNA delivers maximal performance in a range of applications, including real-time PCR. To find out more about the miRNeasy FFPE Kit and QIAGEN's comprehensive range of miRNA research technologies, please visit the link below.

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