International Scholarships and Financial Aid

Sunday, 11 May 2014

How to make 1x BSA


How to make 1x bsa or Bovine serum albumin (BSA)

1x BSA generally suggest that you want  make 1 gram per litre BSA solution.

You can use the following method to make
Material

Phosphate buffered

saline (PBS), pH 7.4,  10X None PS00000041 10X 50 ml 1X
Sigma-Aldrich A4919 Powder 0.5 g 1 g/L 

Preparation:

1. Weigh 0.5 g BSA and add to 300 ml purified water in a 600-ml beaker.
2. Add 50 ml of 10X PBS.
3. Adjust volume to 500 ml.
4. Store at room temperature in an appropriately labeled glass bottle.

No comments:

Science Protocols