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Sunday, 11 May 2014

How to make 1x BSA

How to make 1x bsa or Bovine serum albumin (BSA)

1x BSA generally suggest that you want  make 1 gram per litre BSA solution.

You can use the following method to make

Phosphate buffered

saline (PBS), pH 7.4,  10X None PS00000041 10X 50 ml 1X
Sigma-Aldrich A4919 Powder 0.5 g 1 g/L 


1. Weigh 0.5 g BSA and add to 300 ml purified water in a 600-ml beaker.
2. Add 50 ml of 10X PBS.
3. Adjust volume to 500 ml.
4. Store at room temperature in an appropriately labeled glass bottle.

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