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Sunday, 11 May 2014

How to make 1x buffer from 20x

As explained before here it is very simple and you need to understand the concept.
or you can use the formula M1V1=M2V2


You have 20x Orange Juice and you wanna make 10 ml of 1x.

Simple use M1V1=M2V2

M1= Original Concentration [which is 20x]
V1= required volume to make 1x

M2= Desired Concentration [which is 1x]
V2= Desired volume which is 10 ml

20x * V1 = 1x * 10ml

V1= 0.5 ml of 20x concentrated juice is needed to add in 9.5 ml of water [10-0.5=9.5 ml]


Don't add 0.5 ml in 10 ml. This will make final volume 10.5 ml and your concentration will be wrong. 

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