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Sunday, 11 May 2014

How to make 1x buffer from 10x

This is very simple

You just need to learn the concept.

10x means 10 times concentrated

you need to make 1x

so you need to dilute it 10 time.

of you can take 1 part of 10x and mix with 9 part of water [1+9=10] to make 10x buffer.

Or you can use formula below make 1x buffer from 10x concentration buffer


M1 = stock concentration [10x]
V1 = volume needed of M1 concentrated stock [Z]

M2 = desired concentration [1x]
V2 = desired volume [say 10 ml you wanna make]

So as per M1V1=M2V2

10x * Zml = 1x *10ml

Z =1ml of 10x you need in 10ml of water.

This also means you need to add 10-1=9 ml of water in 1 ml of 10x concentrate to make 1x buffer.


Don't add 1ml in to 10 ml. which would be 11 ml and your concentration will be wrong.

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