Protein Characterization Utilizing Unique New Capabilities from a Dynamic Light Scattering Instrument

Protein Characterization Utilizing Unique New Capabilities from a Dynamic Light Scattering Instrument
The molecular weight of biological materials and polymers can be determined from both static light scattering (SLS) and dynamic light scattering (DLS) experiments using Malvern's Zetasizer Nano. In addition the molecular size of very small molecules in solution can be measured using the Zetasizer Nano, both as a batch measurement in cuvettes, and with the use of a flow cell, as a detector at the end of a chromatography column.
more...

Cell Signaling: Chasing Down Kinases And Their Targets

Cell Signaling: Chasing Down Kinases And Their Targets
Signaling pathways are everywhere. We couldn’t live without them – both the intracellular pathways that govern the interior workings of cells, and the pathways involved in intercellular communication, and the communication of cells with their physical environment. But these nicely organized... more...

No More Slab Gels for DNA and RNA

No More Slab Gels for DNA and RNA
eGene Inc., now a Qiagen company, sells the HDA-GT12TM Genetic Analyzer for the separation and detection of DNA/RNA molecules. The HDA system automatically handles samples in 12-well strips or a 96-well tray, gives DNA resolution up to 2-5 bp, uses less than 1 µL sample volume, and provides DNA size and concentration data in digital format. This analyzer combines automatic gel electrophoresis and accurate gel documentation in one compact system.
more...

Get a head start on your writing this festive season

Get a head start on your writing this festive season

If you're going to give the traditional Christmas telly a miss this year (how many times have you watched reruns of Only Fools and Horses or Mary Poppins!) to instead start work on your final dissertation or making serious headway on your thesis, you might want to think about treating yourself to a tool that will make creating citations and bibliographies a hundred times quicker and simpler. EndNote makes working with literature references easier at every stage, from capturing and organising relevant extracts, to using them in your writing.

Buy and download your copy of EndNote 24/7 (even on Christmas Day) to save the most money.

New EndNote case study - capturing and cataloguing palliative

New EndNote case study - capturing and cataloguing palliative
care research

This case study shows how EndNote helped a team of researchers from the Association for Children's Palliative Care charity (ACT) gather together and organise relevant research to create a bi-annual journal called PaedPalLit. The journal delivers a complete, easy-to-reference guide to the latest palliative care research, providing an invaluable source of information to busy practitioners, clinicians and affected families.

Download your copy of the ACT EndNote case study

Free antibody production

Visit  www.novusbio.com and fill out the Antibody Grants Sheet for a chance to receive 2 mgs of antibody!

Grant Award Date: 1 Award selected on the 15th of every month. Awardees will receive a 0.2 mg test sample of affinity purified rabbit sera produced by Novus Biologicals. (Typical antibody production takes 4-5 months). If the product works and you supply the necessary documentation, you will receive 2 mgs of affinity purified antibody in exchange for product feedback.

Submit by the end of the month to be selected in the following month’s drawing.

---

Sign-up for future news on antibody grants,
new products and technical tips here

From Paraffin Sample to RT-ready RNA in 2 hours

From Paraffin Sample to RT-ready RNA in 2 hours with Maximized Yield

WaxFree(tm) RNA is designed to extract RNA from both formalin-fixed
paraffin embedded (FFPE) samples and fresh and frozen tissue. The
straightforward protocol employs a non-binding method to efficiently
extract RNA. WaxFree minimizes the RNA loss typically observed in
traditional column or bead methods so it is particularly useful for old
or small samples. In addition, most extractions are completed in
approximately two hours without the use of toxic xylene.

Full details here
http://www.trimgen.com/product_rna.asp

PCRMatch.com Bio-Rad PCR and real-time PCR tools

Bio-Rad is committed to providing the best tools for PCR and real-time PCR. Register now on PCRMatch.com and receive the information you'll need to find your perfect match.

Bio-Rad offers the most complete line of thermal cyclers available. With Bio-Rad cyclers
you can:

Quantitate up to 5 targets per well Optimize annealing temperature in a single run Increase success with enzymes that work where others fail Improve reliability

Register now at www.pcrmatch.com. Your perfect match is out there!

Need more information? Visit the interactive PCR Doctor at www.bio-rad.com/genomics/support to define your PCR needs and learn the PCR tip of the week

Trypsin Recombinant, Proteomics Grade

Trypsin Recombinant, Proteomics Grade
Roche's Trypsin Recombinant, Proteomics Grade is a highly purified protease especially designed for the digestion of proteins separated by 2-D gel electrophoresis or liquid chromatographic methods in the course of sample preparation for mass spectrometry. The enzyme is free of contamination from other proteases, and features high specific activity and a reproducible cleavage pattern, leading to faster digestions and more accurate identification of proteins in mass spectrometry. Roche's broad range of sequencing-grade proteases also includes Lys-C, Glu-C, Asp-N, Arg-C, Chymotrypsin, and Carboxypeptidase Y.
more...

Human Retinol-binding Protein 4 QuantikineR ELISA

Human Retinol-binding Protein 4 Quantikine(r) ELISA
R&D Systems now offers a human Retinol-binding Protein 4 (RBP4)
Quantikine ELISA (Catalog # DRB400 ). RBP4 binds to and transports
retinol (vitamin A) from the liver to the peripheral tissues. RBP4 also
promotes hyperglycemia through downregulation of the glucose transporter
GLUT4 in adipocytes, the upregulation of the hepatic gluconeogenic
enzyme PEPCK, and the attenuation of insulin receptor signaling in
skeletal muscle. Serum RBP4 levels are also reported elevated in type 2
diabetes and obesity. This new ELISA is designed to measure RBP4
concentrations in cell culture supernatant, serum, plasma, urine, and
saliva. For more information, please visit www.rndsystems.com or call
1-800-343-7475.

Atlas Antibodies - 1000 NEW Products

 

	1,000 NEW HPA Antibodies More than 700 immunohistochemically (IHC) stained tissue and cell sample images available for each HPA Antibody Immunofluorescence (IF) staining images available for more than 800 HPA Antibodies More than 1,000 Western Blot (WB) images available for more information please visit us at: www.atlasantibodies.com

 

Quick Blocking for Perfect Westerns

When the milk gets old, it's time to throw it away. Our 5M QuickBlock Kit can completely block any solid surface in just five minutes, reaching the same level of antigen detection with smaller amounts of antibody. http://www.biologyproject.net/quick_block_kit.html · Quick procedure: Efficiently blocks a western blot or ELISA plate in only five minutes. · Increased sensitivity: Significantly increases the ratio of signal over background noise, thus requires much less amount of antibody. · Wide range: Works with over 80% of the antibodies from the most popular species · Easy optimization: Determines the best blocking reagents for problematic antibodies · Low cost: The kits are designed only for blocking. There are no bundled reagents to inflate the price and depress your budget. Our customer representatives are available 24 hours, Monday through Friday. You may contact us anytime for assistance. Please check our contact page http://www.biologyproject.net/contact.html for our ocal reginal numbers.Western Blot Specialist Gen Script Corporation – VWR Strategic Partner 120 Centennial Ave., Piscataway, NJ 08854 Tel: 1-732-885-9188 ext 128 Fax: 1-732-210-0262

Custom Peptide Synthesis

Custom Peptide Synthesis

With almost 10 years experience in solid phase peptide synthesis, SBS Genetech is committed to supplying high quality peptides at competitive prices and providing our customers with the extensive services and products:• Purities from crude to 98% • Acetylation/Amidation • Phosphorylation/Fluorescein/Biotin labeled peptides • Cyclic peptides • KLH/BSA Conjugation • MAP peptides• 2-3 weeks turn-around time.

Check the price here
http://www.biocompare.com/optin/20071129_2077.html

Purification of miRNA from FFPE Sections

Purification of miRNA from FFPE Sections

The miRNeasy FFPE Kit from QIAGEN enables purification of total RNA that includes miRNA and other small RNA from formalin-fixed, paraffin-embedded (FFPE) tissue sections including laser capture microscopy samples. Fixing tissues with formalin leads to RNA–RNA and RNA–protein crosslinking, which impairs RNA performance in downstream applications. The miRNeasy FFPE Kit reverses formalin crosslinking and releases RNA from tissue sections while avoiding further RNA degradation. Optimized binding conditions allow purification of all usable RNA from approximately 18 nucleotides upwards. The purified RNA delivers maximal performance in a range of applications, including real-time PCR. To find out more about the miRNeasy FFPE Kit and QIAGEN's comprehensive range of miRNA research technologies, please visit the link below.

miRNeasy FFPE Kit

New online tool for LC method transfer

Better LC results, faster than ever.
Calculate it for yourself and compare
The Agilent 1200 Series Rapid Resolution LC (RRLC) system lets you keep
all your options open. With just one system you can push RRLC
performance to new limits and continue to run your conventional LC
methods, too.

Agilent's new online tool for LC method transfer
and cost calculation lets you make your own calculations and immediately
see the results:

Transfer your conventional LC methods
to RRLC methods
Compare equipment, consumables and
labor costs for conventional LC to RRLC
Try out the new Agilent LC method translator
plus cost calculator tool www.agilent.com/chem/RRLC_MT

Nanoparticle siRNA Transfection System

 

 

 

N-TER™ Nanoparticle siRNA Transfection System: siRNA Delivery to Difficult-to-Transfect Cells

Traditional lipid-based siRNA transfection reagents have a number of drawbacks, including a limited ability to transfect into a variety of cell types, such as primary, neuronal, differentiated, and non-dividing cells. Sigma's N-TER™ Nanoparticle siRNA Transfection System is a peptide based transfection reagent specifically designed to bypass these limitations and allow for efficient delivery of siRNAs into historically unapproachable eukaryotic cell lines. For more information, please visit us online at the link below.
more...

Novel Luciferase Assay System for HTS Applications

Targeting Systems Offers a Novel Luciferase Assay System for HTS Applications
This product is based on gaussia luciferase, a secreted luciferase that is more than a 1000 times brighter than firefly or renilla luciferases. When it comes to screening compounds that affect the activity of a weak promoter, an assay type and readout method can make the difference between actually finding compounds or proteins of interest and not finding them. Gaussia luciferase is extremely attractive for drug screening involving weak promoters. Ease of use, elimination of cell lysis, signal stability, and high signal intensity make the Secreted Gaussia Luciferase System a powerful tool for high-throughput applications. Targeting Systems also offers a dual luciferase reporter system based on gaussia luciferase with firefly luciferase as the second reporter.
more...

Reproducibility in Protein Biomarker Research WEBINAR

Reproducibility in Protein Biomarker Research WEBINAR

Please join our panel of experts on 5 December 2007 for a live online
educational seminar, "Tackling Reproducibility Issues in Mass
Spectrometry-based Biomarker Discovery."

Submit your questions LIVE to the experts during the webinar!

Date: Wednesday, December 5, 2007
Time: 12:00 noon EST; 9:00 am PST; 5 pm GMT
Duration: 1 hour

Register now! For more information and complimentary registration visit:

www.sciencemag.org/webinar

Mass spectrometry is an extremely powerful and sensitive technology that
can detect very small changes in expression levels. Some of these
changes may stem from the biological differences related to a disease or
treatment of interest. Others, however, may reflect the heterogeneity of
patients across multiple sites, the inherent biological complexity and
diversity of different sample types, and even small differences in
sample collection, processing, handling, and analysis techniques used by
multiple operators across multiple locations. As a consequence, data may
be tainted by site-, study-, population-, or sample-specific anomalies
and, therefore, not be sufficiently robust for biomarker discovery.

Join our panel of experts in a live, audience-driven Q&A as they discuss
how to overcome these reproducibility issues to generate less biased and
more reliable results. Questions for the panel can be submitted live
through your viewing console.

Participants:

Martin Latterich, Ph.D.
Faculty of Pharmacy
University of Montreal
Montreal, Canada

Timothy D. Veenstra, Ph.D.
SAIC-Frederick, Inc.
National Cancer Institute at Frederick
Frederick , MD

Toni Whistler, Ph.D.
Chronic Viral Diseases Branch
Centers for Disease Control and Prevention
Atlanta, GA
REGISTER NOW: www.sciencemag.org/webinar

--- Produced by the Science /AAAS Business Office and sponsored by
Bio-Rad ---

2007-2008 Sigma Antibodies Catalog

Introducing the 2007-2008 Sigma Antibodies Catalog
This new 320-page catalog includes:
* Over 4,000 high-quality antibodies
* Monoclonals, polyclonals, secondaries & conjugates
* Over 750 new antibodies
* Application photos
* Associated gene symbols
* Technical appendices
Click here to request your free copy online at
http://www.sigma.com/stkeabcatalog

BULL'S-EYE-InvitrogenT GPCR research tools

LESS MOVING TARGET, MORE BULL'S-EYE-Invitrogen(tm) GPCR research tools

Gain a clear advantage when interrogating difficult receptors with
integrated GPCR cellular assays from Invitrogen. The expert tools needed
to see the most lucrative targets, including target assays with multiple
readout options, are right in front of you. Learn more and sign up for
the Drug Discovery E-newsletter at www.invitrogen.com/gpcr.

2008 AAI Annual Meeting Alert

 

2008 AAI Annual Meeting Alert Only 6 days to Abstract Deadline! Only 13 days to AAI Awards Deadline!   EB 2008 Abstracts Due Wednesday, November 7! Click here to submit    AAI  Award Nominations, Applications Due Wednesday, November 14! Click here for details FOR 2008!  AAI Trainee Abstract Awards!   Due Wednesday, November 7!   AAI Annual Meeting Preliminary Program The American Association of Immunologists (AAI) 9650 Rockville Pike * Bethesda, MD 20814 301-634-7178 * infoaai@aai.org

analyze gene expression or DNA methylation

Do you knock down genes with siRNA, analyze gene expression or DNA methylation, or study miRNAs? We understand the major challenges that have to be overcome.

 

Our sample and assay technologies ensure that you obtain the reliable, high-quality data you need. From sample collection and stabilization to analyte purification and analysis - we’ve got it covered.

 

For more details, visit our application pages:

• Gene Expression Analysis
• Gene Silencing
• miRNA Research
• Epigenetics

 

You can also find out more in our new brochure Analyzing Gene Expression and Regulation.

PCR.ppt- Microsoft PowerPoint

[PDF]

Microsoft PowerPoint - PCR.ppt

File Format: PDF/Adobe Acrobat - View as HTML
Nucleotides. Primers. polymerase. PCR is DNA replication in a test tube ... Reverse trancriptase PCR. Use mRNA as a template. Isolated cDNA clones ...
learn.sdstate.edu/chend/Courses/2006.Fall/Math592.Bioinformatics/PCR.pdf -

ARK-Genomics conference 2008: 3rd International Symposiumon Animal Functional Genomics (ISAFG)

ARK-Genomics conference 2008: 3rd International Symposium on Animal Functional Genomics (ISAFG)

Dates:
April 7th – 9th 2008

Venue:
Edinburgh International Conference Centre (EICC), Morrison Street, Edinburgh, UK

Official Conference Website:
http://ebrc-launch.org/ISAFG/index.html
(updated information about agenda, registration, abstract submission, accommodation and venue)

Registration and Abstract Submission will open on Friday, October 12^th 2007
Reduced rates for PhD and first year post docs available.
Limited number of travel bursaries for young researchers available.

Highlights:
The 3rd International Symposium on Animal Functional Genomics (ISAFG) will present opportunities to take stock of the current European and International position in animal functional genomics, identifying areas for collaborations and the development of new resources.

Increased exposure through the bringing together of two important conference series: 
1st European Farm Animal Functional Genomics Conference (ARK-Genomics) and
2nd International Symposium on Animal Functional Genomics (Michigan State University)

Themes:
The conference will be structured around the following themes:
“Genetics and QTLs”

“Bioinformatics and Data Mining”

“Animal Health”

“Functional Genomics meets Physiology”

“Animal Genomics in Industry”

“Proteomics”

“Systems Biology”

The ISAFG event will run in parallel with the launch of the EBRC, a new world class animal bioscience centre.
The keynote lectures by Professor John Quackenbush and Professor Michel Georges will highlight functional genomics and its impact on Systems Biology.

Additionally there will be 2 open sessions, a poster session with cheese &wine as well as a conference dinner.
As before, there will be plenty of opportunities for networking.

More information about ARK-Genomics, the collaborative functional genomics research centre can be found at:

http://www.ark-genomics.org/

 

RNA In Situ Hybridization Protocol

96-well RNA In Situ Hybridization Protocol - http://www.fruitfly.org/about/methods/RNAinsitu.html

96-well RNA In Situ Hybridization Protocol. Probe Preparation, Cell Inoculation, PCR, RNA Probe Preparation, etc, Very In-depth Protocol and Method Conditions. Berkeley Drosophila Genome Project.

http://www.fruitfly.org/about/methods/RNAinsitu.html

Milestones in DNA Technologies

Milestones in DNA Technologies

A collaboration from Nature, Nature Methods and Nature Reviews Genetics, Milestones in DNA Technologies will focus on ground-breaking technologies and advances in the analysis of DNA. It will contain articles from the participating journals and original commentaries written by personalities who have witnessed the unfolding, or consequences, of these milestone achievements.

Free print copies available! Click here to request your free print copy.

Climate Scientists, Gore Share Nobel Peace Prize

Climate Scientists, Gore Share Nobel Peace Prize
Committee honors efforts to spread global awareness of climate change

http://sciencenow.sciencemag.org/cgi/content/full/2007/1012/1?etoc

Neuron SFN Satellite Meeting: Register Now!

Only 21 days until the meeting... Register to attend and Submit your Abstract today!

Neurons on the Map (just preceding the SfN meeting)
November 1, 2007
San Diego, CA

For the complete meeting details, visit http://www.neuron-meeting.com.

If you have any questions regarding registration, please contact Charlotte Wilkins c.wilkins@elsevier.com.

Viral Metagenomics WEBINAR

Viral Metagenomics WEBINAR

Join an online seminar on 24 October 2007 entitled "A Viral Metagenomics Study of Honey Bee Colony Collapse Disorder" presented by two eminent authors of a recently published Science paper.

Submit your questions LIVE to the experts during the webinar!

Date: Wednesday, October 24, 2007
Time: 12:00 noon EDT; 9:00 a.m. PDT ; 5 p.m. GMT
Duration: 1 hour

Register now!
For more information and complimentary registration visit:
www.sciencemag.org/webinar


Colony collapse disorder (CCD) among honey bee populations in the United States has resulted in the loss of between 50% and 90% of hive colonies. Previous studies have pointed to the possibility that an infectious agent could be involved. A recent study published in Science magazine used unbiased metagenomic analysis to survey microflora present in normal and CCD-affected hives to determine whether a pathological agent could be linked to CCD. The authors found that the presence of one virus, Israeli acute paralysis virus of bees (IAPV), showed a strong correlation with colony collapse disorder. In addition to the important economical implications, this work also represents a novel use for massively parallel next generation sequencing technology which has enabled this type of high level metagenomic study.

You will hear our panel, which includes two of the study's authors, discussing:
- how metagenomics can be applied in the discovery of unknown pathogens
- the importance of study design and data analysis in metagenomics research
- how recent technological advances have made this type of study possible

Participants:
W. Ian Lipkin, M.D.
Professor of Epidemiology and
Professor of Neurology and Pathology
Mailman School of Public Health
Columbia University
New York , NY

Michael Egholm, Ph.D.
Vice President of Research & Development
454 Life Sciences
Branford , CT

REGISTER NOW AT: www.sciencemag.org/webinar

Buy EVOcard to Receive Christmas presents

Buy your EVOcard and receive 2 Christmas presents on orders placed until 24th December for oligos & sequencing
Up to 35% discount off the list price on your MWG orders (35% discount on unmodified oligonucleotides; 15% discount on sequencing reactions and modified oligonucleotides) Amazon vouchers or EVOcard bonus (7.5% of total order spend until 24/12/07 arwarded as Amazon vouchers; 15% of total order spend from until 24/12/07 applied to account as EVOcard bonus) Please register by email to be eligible for the bonus - send contact details and your preference of EVOcard bonus or Amazon vouchers to evocardpromotion@mwgdna.com or faxback this postcard to + 49 80928289970.

Millipore Cell Signaling Application Guide

Invaluable, up-to-date information on one of the most critical life science workflows—Cell Signaling—is now available.
Are you looking for the latest products and technical information in cell signaling? With more than 500 pages of products, workflows and technical reference material the New Cell Signaling Application Guide provides the most comprehensive support and advanced selection of tools available in the area of cell signaling.

The Cell Signaling Application Guide includes dozens of cell signaling pathways, over 100 pages of technical articles, an extensive troubleshooting section, as well as thousands of Upstate cell signaling products including detailed product information in the following areas: • Upstate enzymes, antibodies and kits • Epigenetics and Chromatin Function • Multiplexing and Drug Discovery Services • Pathway Diagrams

Request your copy of the Cell Signaling Application Guide.

Real-time PCR Video for GM soybean DNA

PCR Animation

PCR Tutorial Animation

Molecular Structure Animation

Interleukin-1 binding to its receptor on a cell surface, created from structural data

SDS-PAGE VIDEO

Biochemical Applications 101 - Sodium dodecylsuplhate gelelectrophoresis (SDS-PAGE)

Video SDS PAGE

A video showing SDS polyacrylamide gel electrophoresis in ~120x time (constant voltage). Note the stained markers on the right.

Gel Electrophoresis and Blotting 2

Gel Electrophoresis and Blotting video

James giving a talk at Hunter College on Gel Electrophoresis and Blotting.

Receive 50% off new GPCR division arrested cells

Receive 50% off new GPCR division arrested cells
Invitrogen's Division Arrest (DA) technology for GPCRs allows you to use frozen cells as ordinary, cost-effective assay reagents for screening. Now for a limited time you can choose from more than 40 of our GeneBLAzer^® GPCR division arrested cells at 50% off the list price - simply quote P392640 when you place your order.
Sample data for selected targets can be obtained by clicking in the table below.

H1
H1-NFAT-bla HEK293T
K1299
H1.pdf

H2
H2-CRE-bla HEK293T
K1307
H2.pdf

EDG3
EDG3-Ga15-NFAT-bla HEK293T
K1319
EDG3.pdf

EDG7
EDG7-NFAT-bla HEK293T
K1315
EDG7.pdf

D2
D2-Gqo5-NFAT-bla CHO-k1
K1309
D2-Gqo5.pdf

View the complete list of GeneBLAzer^® Division Arrested GPCR cells.

Stem Cell Primer

The Politics of Stem Cell Research

Get Developmental Cell for $99!

SPECIAL OFFER:
Get Developmental Cell for $99!

Your 1-year personal print subscription includes:

• 12 monthly issues, filled with fundamental advances in the fields of cell and developmental biology;
• Reviews, commentaries and analyses complementing the issue's primary research papers; and
• Free, full-text online access to Developmental Cell (including early publication material and the online archive).

Subscribe for $99 today! (When prompted for a Discount Code, enter i00307.)

New Qubit® fluorometer from Invitrogen

Are DNA/RNA quantitation, time, and money related? With accurate
quantitation, you decide how much DNA/RNA to use in experiments—
giving more reliable results. The Qubit® fluorometer is more
accurate than UV absorbance, saving time and money. Try our demo at
www.invitrogen.com/qubitsavesyoumoney.

Science Podcast

Science Podcast
Join host Robert Frederick for interviews and stories on attaching cameras to birds to study their behavior; a downside to breeding fish in captivity; making your lab and scientific conference environmentally friendly; and more. It's all free. Go to:
http://www.sciencemag.org/multimedia/podcast/#20071005.

NEW! Cell's Latest Podcast

NEW! Cell's Latest Podcast

In our newest podcast, Dr. Emilie Marcus talks to Dr. Rudi Grosschedl about how gene regulation can be coordinated by interactions between chromosomes, and we hear from Dr. Jim Collins about a new way in which common antibiotics can kill bacteria. We also learn about some of the exciting research published in Cell in the last few months, including a study that shows how bones can keep you slim and give you energy. Listen to Cell's Podcast.

Western analysis in one day

Tough protein separation?
Get more novel, more Novex(r). Do a Western analysis in one day,
efficiently analyze native proteins, transfer proteins in 7 minutes.
Novex(r) products bring it all together. Isn't time your protein
analysis was as novel as your thinking? Get novel at
www.invitrogen.com/NOVEX.

SilenciX cell lines-Already silenced stable cell lines

SilenciX cell lines
Unique in Europe: Already silenced stable cell lines
Our pre-silenced SilenciX cell lines is a breakthrough in knock-down expression. Such
catalog product is only available at tebu-bio.
Don't wait anymore to benefit from our first stable silenced cell system:
*   Extinction already performed: from shRNA design to validation by qPCR, what
    you just need to do is to thaw the cells!
*   Over 70% knockdown guaranteed
*   Long-term stability > 500 days
*   Control included: you receive 1 vial of "target specific" SilenciX cells validated
    by qPCR and 1 vial of control SilenciX cells (transfected with a plasmid encoding
    for a non-relevant sequence).
*   No virus infection: No particular handling safety is needed
*   For 4,800 € only (£ 3,265)
    Your target does not appear in our current list? We will put it at the top of our
    production plan.
Customization for cell types (human) and targets is also possible. For further
information, you can contact our product specialist  Aurélie SERRA.
www.tebu-bio.com: the smarter way to buy research products

ClonePix FL

ClonePix FL: powerful technology to explore cell surfaces and discover stellar clones. Select and isolate mammalian clones by cell surface protein expression, rapidly yielding monoclonal populations for cell based assays, screening and discovery research. Significantly improve timelines, labor costs and efficiency of cell line selection.

Nuclear Receptors in Liver and Digestive Diseases: A Research Workshop

Sponsored by: Division of Diabetes, Endocrinology, and Metabolic Diseases (DEM) and
Division of Digestive Diseases and Nutrition (DDN)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)

Description of the Meeting:

The nuclear receptor (NR) superfamily of transcriptional regulators has been integrally involved in the regulation of a variety of genes related to such diverse cellular and systems processes as the control of development to the regulation of bile acid and cholesterol biosynthesis. Disruption of the NR signaling pathways has been implicated in a variety of disease states such as diabetes, obesity, liver diseases such as non-alcoholic steatohepatitis, and other endocrine-related disorders. Many of the NR-implicated liver diseases are areas of high priority for research as outlined in the Trans-NIH Action Plan for Liver Disease Research (http://www.niddk.nih.gov/fund/divisions/ddn/ldrb/ldrb_action_plan.htm). Additionally, the NIDDK has recognized the importance of NRs in a variety of disease states and currently supports the Nuclear Receptor Signaling Atlas (http://www.nursa.org/index.cfm), an open access web portal for information about the NR superfamily.
This meeting will bring together scientists focused on the mechanisms and basic science of NR transcriptional regulation and translational and clinical investigators focused on the pathophysiology of digestive and liver disease states where an NR pathway has been implicated. Two keynote talks will cover the current basic understanding of NR regulation and the potential role of NR signaling dysregulation in the pathophysiology of disease conditions. Other talks will explore the known physiologic roles of ligands and receptors, such as sterol regulatory element-binding protein (SREBP), peroxisome proliferator-activated receptor (PPAR), retinoid X receptor (RXR), liver X receptor (LXR), and small heterodimer partner (SHP), and their downstream signaling in normal and in specific clinical conditions. The signaling mechanisms implicated in the development of obesity, non-alcoholic steatohepatitis, diabetes, bile acid and cholesterol synthesis dysregulation, and so on, also will be explored.
The aim of this meeting is to review the present state-of-the art knowledge of NRs, to promote cross-fertilization among the spectrum of basic, translational, and clinical investigators, and to integrate the current understanding of NR biology and the current clinical challenges for a variety of digestive and liver disease states. At the conclusion of the meeting, the organizers will define research challenges identified during the meeting. A meeting summary manuscript, including the research challenges identified, will be submitted for publication.

Organizers:

Edward Doo, Liver Disease Research Branch (LDRB), DDN, NIDDK
Ronald Evans, The Salk Institute for Biological Studies
Saul Karpen, Baylor College of Medicine
Joel Lavine, University of California at San Diego
Ronald Margolis, DEM, NIDDK
Patricia Robuck, LDRB, DDN, NIDDK

Locked Nucleic Acid-based arrays

The next generation of Exiqon's popular Locked Nucleic Acid-based arrays - the miRCURY^TM LNA microRNA Array - is now available. With unmatched specificity, you can conduct expression profiling of a comprehensive range of microRNAs from any organism you like - vertebrates, invertebrates, plants and viruses, with unmatched sensitivity (sample from just 30 ng total RNA). You even have access to 150 proprietary microRNAs unavailable elsewhere (miRPlus^TM). Our microRNA array platform is part of Exiqon's miRCURY^TM product line, the most complete range of tools for microRNA analysis available today. The miRCURY^TM tools enable you to seek, find and verify microRNAs - and to accelerate your discoveries.
www.exiqon.com/array-embo

Invitrogen Cellular Analysis 2007

The 2007 catalog from Invitrogen Cellular Analysis presents a broad portfolio of enabling inventions and their applications, brought together specifically to meet the challenges of examining biology in context. Order your copy today at www.invitrogen.com/cellsrock

Neuroscience Recruitment Advertising Package

Are you recruiting for researchers, post docs, faculty or staff in the field of neuroscience?

Satisfy your recruitment needs with our Neuroscience Recruitment Advertising Package!
Your advertisement will be featured in print in New Scientist magazine, the Neuron Special Supplement on Neuroscience Jobs & Trends, and online on ScienceJobs.com! Contact us at nssales@elsevier.com to learn about our discounted package rates and bonus distribution to the SfN Annual Meeting.

Transfection Research Award

Celebrate 10 Years of FuGENE® Reagents in Washington, D.C. in December!
Don't miss the chance to apply for the Transfection Research Award, and be eligible to win a trip to present your FuGENE® data at the 2007 ASCB Annual Meeting in Washington, D.C.!
Visit 10th Anniversary website for details and more information.

Additional GPCR Expressing Cell Lines Released

Lonza Releases Four New GPCR Expressing Cell Lines

 

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EBook-Clinical Chemistry: A Laboratory Perspective

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Ebook Biology in the Laboratory: (3rd edition)

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eBook Mathematical Methods in Natural Science

Mathematical Methods in Natural Science

This book provides a general introduction to applied analysis; vector analysis with physical motivation, calculus of variation, Fourier analysis, eigenfunction expansion, distribution, and so forth, including a catalogue of mathematical theories, such as basic analysis, topological spaces, complex function theory, real analysis, and abstract analysis. This book also gives fundamental ideas of applied mathematics to discuss recent developments in nonlinear science, such as mathematical modeling of reinforced random motion of particles, semi-conductor device equation in applied physics, and chemotaxis in biology. Several tools in linear PDE theory, such as fundamental solutions, Perron's method, layer potentials, iteration scheme, are described, as well as systematic descriptions on the recent study of blowup of the solution. Link

eBook Neuroscience and Philosophy: Brain, Mind, and Language

 

Neuroscience and Philosophy: Brain, Mind, and Language

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Of Pages:number 232

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Writing and Presenting in English: The Rosetta Stone of ScienceThe Rosetta Stone of Science is a useful and practical guide to presenting scientific research in the English language. It is written specifically for scientists who would like to improve the effectiveness with which they use the English language and improve their communicative skills in order to become published and develop more confidence in presenting their work at international conferences.

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Principles and Practice of Pharmaceutical Medicine Ebook

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Tricine-SDS-PAGE Protocol

Tricine-SDS Page Protocol

The following reagents are needed in advance:

Gel monomer (30% acrylamide, 29:1). 29.0 g acrylamide and 1.0 g bisacrylamide are dissolved in a total volume of 100 mL MQ H2O. Store at 4 °C, protect from light.

Gel Buffer. Dissolve 181.7 g Tris base and 1.5 g SDS in 400 mL MQ H2O. Adjust the pH to 8.45 using concentrated HCl. Bring to a final volume of 500 mL with MQ H2O. Store at 4 °C.

Anode Buffer (Lower Buffer). Dissolve 12.11 g Tris base in 400 mL MQ H2O. Adjust to pH 8.9 with HCl. Bring to 500 mL with MQ H2O. Store at 4 °C

Cathode Buffer (Upper Buffer). Dissolve 6.055 g Tris base and 8.96 g Tricine in a total volume of 500 mL MQ H2O. Add 0.5 g SDS. Store at 4 °C.

NOVEX 2X Tricine-SDS sample buffer. For 10mL, mix the following:

3.0 mL 3.0 M Tris-HCl, pH 8.45
2.4 mL glycerol
0.8 g SDS
1.5 mL 0.1% Coomassie Blue G
0.5 mL 0.1% Phenol Red
Distilled water to 10 mL
Other Reagents Needed: TEMED (pure liquid), 10 % (w/v) ammonium persulfate (made freshly), 0.1% (w/v) SDS, and glycerol.

Gel Preparation

Prepare & Pour the Separating Gel: Note - enough for four Novex 1.0 mm mini-gel cassettes. This recipe is for a 12.0 % running gel. In a disposable 50 mL conical tube, mix the following:

12.0 mL of gel monomer.
10 mL of gel buffer
3.1 mL glycerol
4.8 mL MQ H2O
0.1 mL 10% ammonium persulfate.
Iniatiate polymerization by adding 40 uL of TEMED. Transfer to the gel cassettes; bring to approximately 1.5 cm from the top of gel blank (which is reserved for the stacking gel) and gently overlay with a small volume (ca. 0.5 mL) of 0.1% SDS. Polymerize for 30 min. Remove layer of SDS after the gel has polymerized.

Prepare & Pour the Stacking Gel: Note - enough for four Novex 1.0 mm mini-gel cassettes. This recipe is for a 4.0 % stacking gel. In a disposable 50 mL conical tube, mix the following:

1.25 mL of gel monomer
2.5 mL of gel buffer
6.25 mL H2O
0.05 mL 10% ammonium persulfate
Iniatiate polymerization by adding 20 uL of TEMED. Transfer to the gel cassettes and insert comb. Clamp along the top with binder clips to insure well formed wells. Polymerize for 30 min.

Running, Developing and Drying the Gel.

Sample Preparation Samples are prepared by mixing a equivalent volume of sample with an equivalent volume of NOVEX 2X Tricine-SDS sample buffer. The samples are then heated for 5 min in a boiling water bath, centrifuged for one minute,

Gel Setup and Run Parameters. Prior to loading, fill the upper buffer chamber with cathode buffer and the lower chamber with 250 mL of anode buffer. Electrophorese at constant current - 15 mA until the samples have migrated through the stack; 30 mA for the remainder of the run (until the tracking dye has migrated out from the lower slot).

Staining. Staining is accomplished by soaking the gel in a solution of 45% methanol, 45% deionized water, 10% acetic acid, and 0.25% (w/v) Coomassie Blue R-250 on a table top shaker for 30 minutes. The gel is then destained by soaking in a solution of 45% methanol, 45% deionized water, and 10% acetic acid on a table top shaker. Periodically replace with fresh solution until desired destaining is achieved.

Gel Drying. After destaining, the gel is prepared for drying by soaking it for five minutes in gel drying solution (5% glycerol/30% methanol). Next, soak a single piece of cellophane in the same gel dry solution for 30 seconds. Lay down the cellophane first, and the gel on top of this. Next soak the second piece of cellophane in gel dry solution for 30 seconds, and then overlay this on the gel to make a sandwich. Squeeze out any air bubbles and then clamp on upper frame. Dry at room temperature.

Other Protocols Links:

Tricine–SDS-PAGE

Hermann Schägger¹

http://www.nature.com/nprot/journal/v1/n1/full/nprot.2006.4.html 

Tricine-SDS-PAGE

Protocol adapted from Schägger, H., and G. von Jagow. 1987. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166:368-379, as modified by Lesse, A. J., et al. 1990. Increased resolution of lipopolysaccharides and lipooligosaccharides utilizing tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis. J. Immunol. Methods 126:109-117.

http://wheat.pw.usda.gov/~lazo/methods/goldberg/tricine.html

Tricine-SDS-PAGE(ref Schagger, H., and von Jagow, G., Analytical Biochem. 1987, 166, 368-379)

http://molecular-neurogenetics.mcgill.ca/PDF/PDF%20Protocols/Tricine_SDS_PAGE.pdf.

 

17.5% Tricine SDS-PAGE Protocol(from Dr. Chen-ya Chien)

http://www-nmr.cabm.rutgers.edu/labdocuments/biochemprotocols/sds.html