RNAi Europe, 20-21 September, Barcelona, Spain
4th annual RNAi Europe conference and exhibition, will be held in Barcelona, Spain. The agenda will include an excellent list of influential speakers, and we are expecting 30+ exhibitors. A tutorial to review RNAi and MicroRNA: Market Landscape and Emerging Opportunities will be held on the 21 September.
http://www.selectbiosciences.com/conferences/rnaieurope07/index.aspx
Semiquantitative RT-PCR analysis to assess the expression levels of multiple transcripts from the same sample
Maria Marone1*, Simona Mozzetti1, Daniela De Ritis1, Luca Pierelli1 and Giovanni Scambia1
1 Department of Gynecology and Department of Hematology. Catholic University, L.go A. Gemelli 8, 00168 Rome. Italy.
* To whom correspondence should be addressed: Maria Marone, Department of Gynecology and Department of Hematology. Catholic University, L.go A. Gemelli 8, 00168 Rome. Italy. Email: maria.marone@tiscalinet.it
Link
from
Jack Vanden Heuvel
Penn State University
Department of Veterinary Science
and Molecular Toxicology Program
Protocol for competitive RT-PCR
This protocols is from the lab of Dieter Kaufmann, Department of Human Genetics, University of Ulm, Germany
This RT-PCR protocol book describes the detail of novel, useful, and
interesting RT-PCR applications.
protocols available for
highly sensitive detection and quantification of gene expression, the in situ localization of gene,
expression in tissue, and the cloning of genes,
as well as for analyzing T-cell clones and the differential expression of genes,
including laser-capture microdissection (LCM),
real-time and quantitative PCR,
microarray technology, cDNA cloning.
Book Info:
Published in 2002
Published by Humana press
Author Joseph OConnell
ISBN 0896038750 Size 4.30MB
WordWeb is a English thesaurus and dictionary for Windows. New Version 5 - now with fast one-click look up, web-reference tabs, bookmarks, Vista support. Highly useful for non-native English speakers, GRE, TOEFL and other examinations.
By Francis D. Murgatroyd, Andrew D. Krahn, George J. Klein, Raymond K. Yee,
Computational Cardiology: Modeling of Anatomy, Electrophysiology, and Mechanics (Lecture Notes in Computer Science)
By Frank B. Sachse
Publisher: Springer
Number Of Pages: 17
Publication Date: 2004-05-27
Sales Rank: 933074
ISBN / ASIN: 3540219072
EAN: 9783540219071
Binding: Paperback
Manufacturer: Springer
Studio: Springer
Paperback: 504 pages
Now an established handbook of principles and techniques, this text develops an understanding of the relevance of four fundamental properties of the analyte: shape, polarity, charge and size, to the three key types of analysis: separation, identification and quantification.
Publisher: Springer
Number Of Pages: 112
Publication Date: 2005-04-06
Sales Rank:
ISBN / ASIN: 3540240128
EAN: 9783540240129
Binding: Hardcover
Manufacturer: Springer
Studio: Springer
Current Protocols in Molecular Biology
Current Protocols in Molecular Biology
ISBN: 047150338X
Author: Roger Brent / Robert E. Kingston / J. G. Seidman / Kevin Struhl / Frederick M. Ausubel / Virginia Benson Chanda / David D. Moore / J.G. Seidman / F.M. Ausubel
Publisher: John Wiley & Sons Inc
Edition: ringbou edition (December 4, 2003)
ISBN: 047150338X
Ring-bound: 1600 pages
URL: /http://www.amazon.com/exec/obidos/redirect?tag=songstech-20&path=ASIN%2F047150338X
Summary:
Current Protocols in Molecular Biology, the first Current Protocols publication, remains the international standard by which all other lab manuals are judged. Basic methods for DNA preparation and isolation, library screening, and sequencing have been joined by more advanced procedures detailing DNA-protein interactions, yeast manipulation, and phosphorylation analyses. From basics to the cutting edge, CPMB is the only resource you need for successful experiments.
DNA Plasmid is isolated from 1-2 ml bacterial cultures by alkali and SDS treatment.
As more species' genomes are sequenced, computational analysis of these data has become increasingly important. The second, entirely updated edition of this widely praised textbook provides a comprehensive and critical examination of the computational methods needed for analyzing DNA, RNA, and protein data, as well as genomes. The book has been rewritten to make it more accessible to a wider audience, including advanced undergraduate and graduate students. New features include chapter guides and explanatory information panels and glossary terms. New chapters in this second edition cover statistical analysis of sequence alignments, computer programming for bioinformatics, and data management and mining. Practically oriented problems at the ends of chapters enhance the value of the book as a teaching resource. The book also serves as an essential reference for professionals in molecular biology, pharmaceutical, and genome laboratories.
Book Properties
ISBN: 0896036537
Title: SV40 Protocols (Methods in Molecular Biology)
Author:
Publisher: Humana Press
MicroRNA Protocols provides diverse, novel, and useful descriptions of miRNAs in several species, including plants, worms, flies, fish, chicks, mice, and humans. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the identification of miRNAs. This volume will stimulate the reader to explore diverse ways to understanding the mechanism in which miRNAs facilitate the molecular aspects of the biomedical research.
pass:lekar
The following protocol is written as an aide in the use of a hemacytometer for determining the concentration of
cells in a suspension.
This book provides detailed protocols for all the latest methodologies used to assure the long-term biostorage of a diverse range of biological materials.
Developed in expert laboratories, the protocols have been painstakingly perfected over the years to provide time-tested, step-by-step instructions that ensure robust and reproducible results. Each protocol deals with the preservation of an organelle, cell, or tissue type, and is accessible even to the nonspecialist because of its cookbook approach. Novel protocols can be readily developed with the help of the "hands-on" Notes sections.
Cryopreservation and Freeze-Drying Protocols is an indispensable reference work for both the individual researcher, and all those who want to establish or improve biostorage systems in their laboratories. Its applications range from microbial culture collections, botanic gardens, and zoos to animal husbandry, aquaculture, medicine, human fertilization, and cell and molecular biology.
Pass: econiches
Product Details
Book Publisher: Benjamin Cummings (11 December, 2001)
ISBN: 0805366245
Book author: Neil A. Campbell, Jane B. Reece
Amazon Rating: 4.5
Book Description:
The Sixth Edition of BIOLOGY by Neil Campbell and Jane Reece builds upon the earlier versions' dual goals to both help readers develop a conceptual appreciation of life within the context of integrating themes, and to inspire readers to develop more positive and realistic impressions of science as a human activity.
The authors have thoroughly updated each of the book's eight units to reflect the existing progress in our understanding of life at its many levels, from molecules to ecosystems. Examples of updated content include the Human Genome Project, the revolution in systematics, HIV as a research model in evolutionary biology, the role of cell-signaling pathways in plant responses, new frontiers in neurobiology, and experimental approaches that are advancing ecology. To assure accurate representation of each field of biology, a team of stellar specialists has worked with the authors in updating every unit.
An innovative design breakthrough ensures that the art is as current as the content. Guided Tour diagrams explicitly guide readers through the more challenging figures, succinctly explaining key structures, functions, and steps of processes within the figure, reducing the need to look back and forth between legend and art. It's as if an instructor were looking over the reader's shoulder and clarifying each part of a figure! Guided Tour commentary is set in blue, making it easy to differentiate these explanations from ordinary labels and keeping the figure itself clear and uncluttered. For college instructors and students.
password: lekar
Blogmania has been global and its roots can be found deep in to the hardcore science. This week's Nature article "Blogger Unit" started another wave of science blogging by describing about the book "The Open Laboratory: The Best Writing on Science Blogs 2006". The Book is written by the a US biologist-blogger Bora Zivkovic. He is one of the highly successful science blogger (Bora's blog*) and actively engaged in promoting science blogging.
The book teach us about the science blogging anthology. Having look at the book preview, I personally find it interesting but don't think its good for new science bloggers. Because blogging is not about scientific writing and publishing research.
People love write a blog because they enjoy personal freedom which we don't have in scientific world...
Anyways enjoy the book...
This thoroughly revised second edition succinctly introduces the physics and function of magnetic resonance imaging. All important and clinically relevant aspects are presented in a clearly structured manner. The emphasis is on practical information including the latest trends and developments that are relevant for MRI in the clinical setting. The opening chapters describe the underlying physical principles of the MR experiment and the basic pulse sequences commonly used in clinical MRI. Other chapters are dedicated to more advanced techniques such as parallel imaging and cardiovascular MR imaging. The book is rounded out by chapters on MR contrast media, artifacts, high-field imaging, and safety concerns. An extensive glossary offers rapid access to the most important MRI terminology. The book is intended for readers looking for an easy to understand and concise introduction to this fascinating yet somewhat complex imaging modality at the beginning of their MRI training.
Oxford Handbook of Clinical and Laboratory Investigation (Oxford Handbooks Series)
By
Publisher: Oxford University Press, USA
Number Of Pages: 838
Publication Date: 2005-11-18
Sales Rank: 847502
ISBN / ASIN: 0198566638
EAN: 9780198566632
Binding: Paperback
Manufacturer: Oxford University Press, USA
Studio: Oxford University Press, USA
Average Rating: 5
Total Reviews: 1
Book Description:
Modern medicine is highly complex and investigations are a key part of the diagnostic process. With major advances in technology there are thousands of clinical and laboratory tests available. This book provides a patient-oriented approach to investigation (first part of book) where key symptoms and signs are described along with tests that may be of value in reaching a diagnosis. The remainder of the book is specialty-centred and provides a comprehensive review of all available tests within a given subject. The book emphasises which tests are of value, when tests are not likely to be helpful, along with pitfalls in the interpretation of results. The aim is of the book is to provide a more rational method of investigation and prevent over-investigation which is expensive for the hospital and unpleasant for the patient. The contributors are all active clinicians who are engaged in medical practice, so appreciate the problems faced by junior doctors. The book should also be of value to senior medical students who will be facing finals examinations, and who will soon be on the wards and responsible for ordering tests on their patients.
pass:rafcm
This pediatric primary care text takes a family centered approach, examining issues from the perspective of the child and the caregiver. Unlike most primary care texts, it blends traditional health care with complementary therapies. Throughout, it identifies needs based on culture and ethnicity and lists community resources-including websites--where families can obtain more information or help. Features include: Case Studies, Clinical Pearls, and Clinical Warnings.
Title: Pediatrics 2007
Author: Paul D., M.D. Chan
Publisher: Current Clinical Strategies
Publication Date: 2006-08-08
Number Of Pages: 128
Pass: MeDiCiNe_DoCtOrS
Lecture Notes: Obstetrics and Gynaecology provides a concise introduction to obstetrics and gynaecology for medical students and junior doctors. Six sections examine the female development from the early years to old age. The text starts with a section on Basic Science. Self-assessment questions are found throughout the text to enhance knowledge and understanding.Part 1 looks at female reproductive anatomy and physiology in detail. Part 2 covers the puberty and menstrual problems of young women, sub fertility, pregnancy prevention and introduces benign diseases, genital tract infections and sexual problems.Part 3 examines the reproductive years. Detailed coverage of the mother and foetus in pregnancy includes problems and diseases in pregnancy, labour and the newborn child development. Part 4 covers the mature woman, and includes abnormal vaginal blood loss, pelvic pain, breast disease and screening for gynaecological cancer.Part 5 discusses the older woman and malignant gynaecological conditions, the menopause and pelvic floor disorders. Part 6 provides an audit of Obstetrics and Gynaecology, looking at statistics in reproductive medicine and gives the answers to self-assessment questions found throughout the text.Lecture Notes: Obstetrics and Gynaecology is written specifically for medical students, junior doctors on foundation programmes, midwives and General Practitioners.Review quotes on Previous Editions"Well edited, concise, and a useful source of information."2nd Opinion - Edinburgh Student Gazette"Thankfully these are nothing like my lecture notes. This book is packed with useful info."Surgo, Glasgow University Medical Journal"A really easy book to read, useful in enabling students to grasp the basics."Northwing - Sheffield Medical Magazine
Lecture Notes: Obstetrics and Gynaecology provides a concise introduction to obstetrics and gynaecology for medical students and junior doctors. Six sections examine the female development from the early years to old age. The text starts with a section on Basic Science. Self-assessment questions are found throughout the text to enhance knowledge and understanding.Part 1 looks at female reproductive anatomy and physiology in detail. Part 2 covers the puberty and menstrual problems of young women, sub fertility, pregnancy prevention and introduces benign diseases, genital tract infections and sexual problems.Part 3examines the reproductive years. Detailed coverage of the mother and foetus in pregnancy includes problems and diseases in pregnancy, labour and the newborn child development. Part 4covers the mature woman, and includes abnormal vaginal blood loss, pelvic pain, breast disease and screening for gynaecological cancer.Part 5 discusses the older woman and malignant gynaecological conditions, the menopause and pelvic floor disorders. Part 6 provides an audit of Obstetrics and Gynaecology, looking at statistics in reproductive medicine and gives the answers to self-assessment questions found throughout the text.Lecture Notes: Obstetrics and Gynaecology is written specifically for medical students, junior doctors on foundation programmes, midwives and General Practitioners. Review quotes on Previous Editions "Well edited, concise, and a useful source of information."2nd Opinion - Edinburgh Student Gazette"Thankfully these are nothing like my lecture notes. This book is packed with useful info." Surgo, Glasgow University Medical Journal "A really easy book to read, useful in enabling students to grasp the basics."Northwing - Sheffield Medical Magazine
Interventional Ultrasound in Obstetrics, Gynaecology and the Breast
Author: Joaquin Santolaya-Forgas; Didier L�mery
Publisher: Blackwell Science
Publication Date: 1998-11-15
Number Of Pages: 276
Average Amazon Rating:
Book Id 38394
Pass: Lekar
General Practice is one of McGraw-Hill Australia's best selling, most reputable and well-established titles. It is the 'Bible' for general practitioners and medical students of community medicine, written by Australia's most respected GP, John Murtagh.
Pass: spiderman
A lifeline for the busy GP, the Oxford Handbook of General Practice covers the whole of general practice. It includes hands-on advice and allows rapid access to information to help with any day-to-day problems which might arise in general practice. The general practice section has been revised to include the new General Medical Services contract and appraisal and revalidation. There are new chapters on complementary medicine, chronic disease management and elderly care and increased emphasis on evidence based medicine, sports medicine and practice in a multicultural society. Pointers to further information for GPs and advice and support for patients, which can be easily accessed from the GP surgery, are included throughout. The layout of the handbook has been redesigned with the use of colour-coding to aid quick reference and additional flow charts, tables and diagrams have been included to make information easier to access.
The 2nd of this popular text emphasizes the fundamental concepts and principles of human immunology that students need to know, without overwhelming them with extraneous material. It leads the reader to a firm understanding of basic principles, using full-color illustrations; short, easy-to-read chapters; color tables that summarize key information clinical cases; and much more-all in a conveniently sized volume that's easy to carry. The New Edition has been thoroughly updated to reflect the many advances that are expanding our understanding of the field.
With more than 700 expert authors from 22 different countries, the Encyclopedia of Immunology, Second Edition is the largest comprehensive reference source of current immunological knowledge available. It provides a broad scope and high level of expertise to the many aspects of the field of immunology and related areas, including microbiology, virology, and parasitology. Arranged into 31 subject areas with extensive cross-referencing and subject indexes in each volume, the Encyclopedia is easy-to-use for virtually any researcher, regardless of his or her field. Concise definitions of the subject area also introduce each entry. The Second Edition includes timely and thorough updates for all articles from the First Edition, more than 60 new entries, a glossary of immunological terms in each volume, a total of 500 figures and tables, and new color plates sections.
Key Features
* Four volumes, each containing a subject index
* Approximately 630 different articles
* More than 700 expert contributors from 22 different countries
* Coverage of 31 different subject areas
* Concise definitions of the subject to introduce each entry
* Further reading lists at the end of each entry
* Extensive cross-referencing
* Entries arranged in a single A-Z list for easy access
* Easy-to-read double-column format
* More than 500 figures to complement the text
* More than 60 new articles
* A glossary of immunological terms in each volume
* A color plate section in each volume
The Immunology Guidebook provides an easily accessible text-reference to the more
up-to-date and difficult concepts in the complex science of immunology. It aims to demystify basic concepts and specialised molecular and cellular interactions. Its 18 chapters offer a logical and sequential presentation where much of the data is displayed in carefully designed tables. This book is intended for immunology students, researchers, practitioners and basic biomedical scientists.
* Tables provide a quick reference to difficult to find immunology data
* A distillate of the latest information on immunogenetics of the human MHC associated with tissue transplantation
* Information boxes featurw related web resources
Teresa Attwood (Author), David Parry-Smith (Author)
Identificationandisolationoflectinnu.pdf
238k
IsolationandCharacterizationofRanaca.pdf
1M
Isolationandpartialcharacterizationo.pdf
896k
Isolationandpropertiesofalectinfromt.pdf
287k
Isolationofalectinfromthepericarpofp.pdf
521k
proteinpurificationmicrotomacro.pdf
1M
PurificationandCharacterizationofanN.pdf
*Please note that these links are from other websites and we do not host these files on our server. Links will be deleted if reported abuse. please email us at laboratory.helpdesk@gmail.com
Cancer is not a single genetic disease but, rather, hundreds of diseases consisting of various combinations of genetic alterations. Many types of genetic alterations contribute to neoplastic transformation. The evidence for this statement has now become common knowledge. In a similar vein, almost everybody believes that genome sequencing is paving the way to a revolution in biology and medicine in general but in oncology in particular. We all mention imatinib and a few monoclonal antibodies when we want to demonstrate that targeted therapies are already more than just a vision. We are, however, still unable to foresee clearly the role
genomics will play in the prevention, diagnosis, and treatment of cancer in the next 10 or 15 years. This uncertainty stems mainly from a dearth of clinical data, and most of the data we do have are retrospective and therefore most probably biased. As stated in its introduction, this book is probably the first of its kind devoted to the genomics of cancer. I approached it with mixed feelings: on the one hand, I was hoping to find answers to some of my questions, mainly because the span of the book reaches from molecular profiling to model systems for discovering and validating drug targets, and from molecularly targeted pharmacology to clinomics. The latter is a term coined by Daniel Von Hoff et al., whose chapter in this book defines clinomics as the application of genomics to patient care. On the other hand, I approached the book with some
skepticism, since the field is moving so rapidly that I was afraid to find once again a book that was already too old or just provided reviews that everyone in the field has already read. But I was pleasantly surprised to find a book that is extremely rich in information and detail but is not
more specialized than necessary. One might criticize certain choices the editors have made -- for instance, providing a discussion of the role of proteomics and genomics in bladder cancer but not in colorectal and lung cancer. This, however, is not very important, since the book was not written for physicians who seek an overview of the latest discoveries in the molecular biology of a specific cancer type. Oncogenomics is very good reading for oncologists who would like to understand what is happening in the laboratory and in the preclinical setting, especially in terms of techniques and research approaches. The book is also worth reading for basic and clinical scientists who intend to plan translational and clinical studies that entail oncogenomics. The essence of the book is to bring together scientists from various specialties and share the same
language, a prerequisite for the next "leap forward." It seems logical that the book concludes with an essay by A.C. von Eschenbach, the director of the National Cancer Institute, who gives his vision of oncology in 2015. Franco Cavalli, M.D., F.R.C.P.
Pass: dmc@lekar
TIGR-GlimmerM: A gene finder derived from Glimmer, but developed specifically for eukaryotes. It is based on a dynamic programing algorithm that considers all combinations of possible exons for inclusion in a gene model and chooses the best of these combinations.
Download Glimmer Via FTP
TIGR-Glimmer: Glimmer are for identification of possible genes for prokaryotes sequences . It is based on interpolated Markov models to identify the coding regions and distinguish them from noncoding DNA.
Download Glimmer Via FTP
TIGR-AAT package(A tool for analyzing and annotating genomic sequences): The AAT package includes two sets of programs, one set (DPS/NAP) for comparing the query sequence with a protein database, and the other (DDS/GAP2) for comparing the query with a cDNA database (Huang et al., 1997).
Download AAT package via FTP
IGR-MUMmer(fast alignment of large-scale DNA and protein sequences):A system for aligning whole genome sequences. Using an efficient data structure called a suffix tree, the system is able rapidly to align sequences containing millions of nucleotides.
Download MUMer Via FTP
Clustal X: Clustal X is a new windows interface for the ClustalW multiple sequence alignment program. It provides an integrated environment for performing multiple sequence and profile alignments and analysing the results. The sequence alignment is displayed in a window on the screen. A versatile coloring scheme has been incorporated allowing you to highlight conserved features in the alignment. The pull-down menus at the top of the window allow you to select all the options required for traditional multiple sequence and profile alignment.
Download Clustax (Windows OS)
EBI-Clustal W is a general purpose multiple sequence alignment program for DNA or proteins.It produces biologically meaningful multiple sequence alignments of divergent sequences. It calculates the best match for the selected sequences, and lines them up so that the identities, similarities and differences can be seen.
Download EBI-Clustal W via FTP
Provides sequence similarity searching against nucleotide and protein databases using the Fasta programs. Fasta can be very specific when identifying long regions of low similarity especially for highly diverged sequences.
Download EBI-Fasta executable via FTP
SigmaPlot software helps you quickly create exact graphs
SigmaPlot graphing software from SYSTAT takes you beyond simple spreadsheets to help you show off your work clearly and precisely. With SigmaPlot, you can produce high-quality graphs without spending hours in front of a computer. SigmaPlot offers seamless M'zoft Office integration, so you can easily access data from M'zoft Excel spreadsheets and present your results in M'zoft PowerPoint? presentations.
I'll say Highly Powerful Software
http://www.systat.com/products/sigmaplot/
part 1 linkpart 2 link
I personally find this BioRad manual very useful in SDS page. I even use this protocol to run Hoofer cabinets..
Synopsis
Part of the successful High-Yield "TM" Series, High-Yield "TM" Biostatistics, Second Edition explains concepts, provides examples, and covers the complete range of biostatistics material that can be expected to appear on the USMLE Step 1. New to this edition are references to evidence-based medicine, and information updated to reflect changes in the current USMLE examinations.
Table of Contents
Descriptive statistics - Inferential statistics - Hypothesis testing - Correlational techniques - Research methods - Statistics in epidemiology - Statistics in medical decision making - Ultra-high-yield review
Extremely popular, this student-friendly text presents the practical areas of statistics in terms of their relevance to medicine and the life sciences. Includes many illustrative examples and challenging problems that reinforce the author's unique and intuitive approach to the subject. The new edition features a new two-color design, examples taken from current biomedical literature, and review questions within each chapter.
Chapman & Hall/CRC
ISBN: 0824723392
640pages
2006-
Pdf-English
size:17,42 Mb
Book Description
A compendium of cutting-edge statistical approaches to solving problems in clinical oncology, this book focuses on clinical trials in phases I, II, and III, proteomic and genomic studies, complementary outcomes and exploratory methods. Cancer Forum called the first edition a "good reference book for statisticians who will be designing and analyzing cancer trials." The second edition includes over 1000 references, more than forty world-renowned contributors, and 300 equations, tables, and drawings. Completely revised while keeping the features that made the first edition a bestseller, this is the best single source for up-to-date statistical approaches to research in clinical medicine.
Features:
Provides a comprehensive discussion of sample size
Explores analytical problems generated by controlling treatment costs and maintaining quality of life
Demonstrates the breadth and depth of current activity in the field of survival analysis
Includes recommendations and pointers for free software that allows you to implement programs
Sets the limits on what can and cannot be concluded from single and multiple clinical trials
Contents
Phase I Trials
Phase II Trials
Phase III Trials
Exploratory Analysis and Prognostic Factors
High-Throughput Data and Bioinformatics
Interpreting Clinical Trials
Handbook of Statistics 18: Bioenvironmental and Public Health Statistics (Techniques and Instrumentation in Analytical Chemistry)
Product Details
�Book Publisher: Elsevier Publishing Company (01 April, 2000)
�ISBN: 0444829008
�Book author: Pranab Kumar Sen
Book Description:
Hardbound. In this volume of the Handbook of Statistics with the primary focus on bioenvironmental and public health statistics, a rather off-beat approach has been taken, wherein biostatistical methods that are relevant to the dissemination of bioenvironmental and public health investigations have been thoroughly emphasised, and placed side by side with the fruitful applications. One aspect of statistical methodology that merits special appraisal is the extent of appropriateness of some standard statistical tools in such non-standard applications, and much of the deliberation in this volume is geared to alternative non-standard and application oriented methodology that have been developed to suit better bioenvironmental and public health studies.
Advances in computers and biotechnology have had a profound impact on biomedical research, and as a result complex data sets can now be generated to address extremely complex biological questions. Correspondingly, advances in the statistical methods necessary to analyze such data are following closely behind the advances in data generation methods. The statistical methods required by bioinformatics present many new and difficult problems for the research community.
This book provides an introduction to some of these new methods. The main biological topics treated include sequence analysis, BLAST, microarray analysis, gene finding, and the analysis of evolutionary processes. The main statistical techniques covered include hypothesis testing and estimation, Poisson processes, Markov models and Hidden Markov models, and multiple testing methods.
The second edition features new chapters on microarray analysis and on statistical inference, including a discussion of ANOVA, and discussions of the statistical theory of motifs and methods based on the hypergeometric distribution. Much material has been clarified and reorganized.
The book is written so as to appeal to biologists and computer scientists who wish to know more about the statistical methods of the field, as well as to trained statisticians who wish to become involved with bioinformatics. The earlier chapters introduce the concepts of probability and statistics at an elementary level, but with an emphasis on material relevant to later chapters and often not covered in standard introductory texts. Later chapters should be immediately accessible to the trained statistician. Sufficient mathematical background consists of introductory courses in calculus and linear algebra. The basic biological concepts that are used are explained, or can be understood from the context, and standard mathematical concepts are summarized in an Appendix. Problems are provided at the end of each chapter allowing the reader to develop aspects of the theory outlined in the main text.
Warren J. Ewens holds the Christopher H. Brown Distinguished Professorship at the University of Pennsylvania. He is the author of two books, Population Genetics and Mathematical Population Genetics. He is a senior editor of Annals of Human Genetics and has served on the editorial boards of Theoretical Population Biology, GENETICS, Proceedings of the Royal Society B and SIAM Journal in Mathematical Biology. He is a fellow of the Royal Society and the Australian Academy of Science.
Gregory R. Grant is a senior bioinformatics researcher in the University of Pennsylvania Computational Biology and Informatics Laboratory. He obtained his Ph.D. in number theory from the University of Maryland in 1995 and his Masters in Computer Science from the University of Pennsylvania in 1999.
Comments on the First Edition. "This book would be an ideal text for a postgraduate course.[and] is equally well suited to individual study.. I would recommend the book highly" (Biometrics). "Ewens and Grant have given us a very welcome introduction to what is behind those pretty [graphical user] interfaces" (Naturwissenschaften.). "The authors do an excellent job of presenting the essence of the material without getting bogged down in mathematical details" (Journal. American Staistical. Association). "The authors have restructured classical material to a great extent and the new organization of the different topics is one of the outstanding services of the book" (Metrika).
Publisher Newnes
Author(s) William DeCoursey
ISBN 0750676183
Release Date 01 January 2003
More than ever, American industry especially the semiconductor industry is using statistical methods to improve its competitive edge in the world market. It is becoming more imperative that graduate engineers have solid statistical know-how, yet engineers in industry typically are not well-prepared to use statistics and they are fuzzy about how to apply statistical tools and techniques. This valuable reference makes statistical methods easier and more accessible to engineers.
Although the book can be read sequentially, like a normal textbook, it is designed to be used as a handbook, pointing the reader to the topics and sections pertinent to a particular type of statistical problem. It contains the following features:
* Covers all major topics treated in a standard college engineering statistics course, but minimizes the mathematical derivations and focuses on practical applications
* Uses real data sets/case studies taken from electronics, electrical engineering, and other engineering fields, such as mechanical and chemical engineering
* Contains numerous software examples using the powerful statistical functions of Excel
Today, mathematics, biology, medicine, and statistics are closing the interdisciplinary gap in an unprecedented way and many of the important unanswered questions now emerge at the interface of these disciplines. Now in its Second Edition, this user-friendly guide on biostatistics focuses on the proper use and interpretation of statistical methods. This textbook does not require extensive background in mathematics, making it user-friendly for all students in the public health sciences field. Instead of highlighting derivations of formulas, the authors provide rationales for the formulas, allowing students to grasp a better understanding of the link between biology and statistics. The material on life tables and survival analysis allows students to better understand the recent literature in the health field, particularly in the study of chronic disease treatment. Biostatistics now includes a companion website to demonstrate the different applications of computer packages for performing the various analyses presented in this text.
* Includes access to a companion website with further examples and a full explanation of computer packages
* Over 40% new material with modern real-life examples, exercises and references
* New chapters on Logistic Regression; Analysis of Survey Data; and Study Designs
* Introduces strategies for analyzing complex sample survey data
* Written in a conversational style more accessible to students with real data
Riddle, Donald L.; Blumenthal, Thomas; Meyer, Barbara J.; Priess, James R., editors.
Plainview (NY): Cold Spring Harbor Laboratory Press ; c1997
Kufe, Donald W.; Pollock, Raphael E.; Weichselbaum, Ralph R.; Bast, Robert C., Jr.; Gansler, Ted S.; Holland, James F.; Frei III, Emil, editors. Hamilton (Canada): BC Decker Inc. ; c2003
LUBERT STRYER is currently Winzer Professor in the School of Medicine and Professor of Neurobiology at Stanford University.
Siegel, George J.; Agranoff, Bernard W.; Albers, R. Wayne; Fisher, Stephen K.; Uhler, Michael D., editors
Philadelphia: Lippincott,Williams & Wilkins ; c1999
The Approved Lists of Bacterial Names was published in the International Journal of Systematic Bacteriology (IJSB 30: 225-420, 1980) and reprinted in book form to provide for the requirements of the Bacteriological Code (1976 Revision) in initiating a new starting date for bacterial nomenclature, 1 January 1980.
* Enzyme should be kept in ice or table-top freezer. 10X reaction buffer, dNTPand primers should be kept in ice after thawing. DNA in H2O is not sensitive as much as others. However, the DNA solution should not be kept at room temp for longer than 15 min.
For 25 �l reaction mixture in a 0.5 ml MFT, Taq polymerase 0.3 �l (1.5 Unit; 0.5 Unit/�l) dissolving in H2O 2.5 �l
GeneAmp 9600(Perkin Elmer Cetus): 10-mer PCR for amplification of random genomic DNA fragments of Cotton
* Enzyme should be kept in ice or table-top freezer. 10X reaction buffer, dNTPand primers should be kept in ice after thawing. DNA in H2O is not sensitive as much as others. However, the DNA solution should not be kept at room temp for longer than 15 min.
1) Number on the top of 200 �l thin-wall tubes.
2) Prepare reaction mixture (for example of 25 �l reaction mixture)
1X conc.
2.5 �l of 10X reaction buffer (PerkinElmer) -
2 �l of 10 mM dNTP (PerkinElmer) 0.2 mM
(or mixture of 0.5 �l of four 10 mM dATP, dCTP, dGTP, dTTP)
2 �l of 2.5 mM random primer (Operon) 0.2 mM
Template DNA 10-30 ng (20 ng is opt.)
DNA+H2O 17 �l
AmpliTaq 0.3 �l (1.5 Unit)
(Voltex the AmpliTaq tube briefly before aliquoting the enzyme)
*Storage: The T-vector should be stored at -20C at all times. When stored in dry form, the T-overhangs will last longer (I don't know how long yet). In solution, it lasts at least a couple of weeks at - 20C.
*Enzymes - The batch of SmaI that is used is particularly critical. Some are contaminated with an endonuclease that removes a few bases from the cloning site. The batch of smaI should be checked before it is used to cut vector for cloning purposes. If bluescript is used, EcoRV can be substituted for sma I.
txpljfg@uabcvsr.cvsr.uab.edu
The PCR product in the LMP can be used for ligations directly, without purification. The ligations take place at room temp on the benchtop. I prepare the vector with minimal digestion (~2hr) then treat it with shrimp alkaline phosphatase(USB). I usually prepare a stock of this vector to have on hand, so I know it is good and will have a low background. You may also want to try using an EcoRV cut vector instead of a Sma cut vector.
One point to note....
We have given up blunt end cloning into Sma I sites where possible. Several people have reported problems with Sma I cut DNA. By choice we clone into EcoRV sites.
Gel size (W X L) Agarous (Rec)
Gel tray (cm X cm) Owl Rec. Buffer (L) 1.0 % 0.7 %
A3 20 X 40 600 400 3.0 L 4.0 2.8
B2 12 X 14 130 100 0.5 L 1.0 0.7
C1 7.6 X 5.1 30 20 0.1 L 0.3 2.7
Note: the size of DNA to be separated needs to be matched to the agarous concentration:
Agarose % Range of separation
0.3 60 - 5 kb
0.6 20 - 1 kb
0.7 10 - 0.8 kb
0.9 7 - 0.5 kb
1.2 6 - 0.4 kb
1.5 4 - 0.2 kb
2.0 3 - 0.1 kb
X-gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside) turns blue when incubated in the presence of b-glactosidase. This gene is on several of the cloning plasmids (especially, on the pUC series and lGT11 vectors). When an inserted piece of DNA is placed in the correct restriction site, the lacZ gene is interrupted and the colony does not turn the media blue (colony we want). Be sure to run controls.
PMB #A
1X 200 ml 1X
10 mM MOPS pH 7.0 210 mg Free acid
230 mg Na salt
10 mM Rubidium chloride 240 mg
Autoclave and store at 4 C
PMB #B
1X 200 ml 1X
10 mM MOPS pH 6.5 350 mg Free acid
85 mg Na salt
50 mM Calcium chloride 1.12 g
10 mM Rubidium chloride 240 mg
Autoclave and store at 4 C
