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Wednesday 6 June 2007

Gel Running Buffer or 1x TBE (Tris-Borate-EDTA) Buffer Recipes

1x TBE (Tris-Borate-EDTA) Buffer

90 mM Tris

  • tris(hydroxymethyl)aminomethane

  • Tris base; FW=121.14

90 mM Boric acid

  • Boric acid; FW= 61.83

2 mM EDTA

  • ethylenediaminetetraacetic acid; FW= 292.24

  • ethylenediaminetetraacetic acid dihydrate

  • ethylenediaminetetraacetic acid disodium salt, or disodium ethylenediaminetetraacetic acid; FW= 372.24

This typically results in a solution close to pH 8.3.

This is often prepared as a 5x TBE stock solution and diluted to 1x or 0.5x as needed.

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