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Wednesday 6 June 2007

Gel Running Buffer or 1x TAE (Tris-Acetate-EDTA) Buffer Recipes

1x TAE (Tris-Acetate-EDTA) Buffer

40 mM Tris

  • tris(hydroxymethyl)aminomethane or Tris base, FW=121.14

20 mM Acetic acid

  • Glacial acetic acid (the typical stock solution) is 17.42 M.

1 mM EDTA

  • ethylenediaminetetraacetic acid; FW= 292.24

  • ethylenediaminetetraacetic acid dihydrate

  • ethylenediaminetetraacetic acid disodium salt, or disodium ethylenediaminetetraacetic acid; FW= 372.24

This typically results in a solution close to pH 7.6.

This is often prepared as a 50x TAE stock solution and diluted to 1x as needed.

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