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Wednesday 6 June 2007

How to make 6X DNA Loading Dye?

6X DNA Loading Dye: This is required to load with your samples in gel electrophoresis for further visualization.

1. Prepare stock solutions:

  • 10 ml of a 2% bromophenol blue stock solution.
  • 10 ml of a 2% xylene cyanol stock solution.
  • 50% glycerol solution.

2. Dilute the stock solutions to prepare 10ml of the final 6X Loading dye with the following component concentrations:

  • 30% glycerol
  • 0.3% bromophenol blue
  • 0.3% xylene cyanol

The 6X loading dye solution can be stored indefinitely in the refrigerator. The bromophenol blue, xylene cyanol, and glycerol stock solutions can be stored indefinitely at room temperature.

The 6X DNA Loading Dye is added to DNA samples to achieve a final dye concentration of 1X.

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