10x PBS Buffer Recipe

10X PBS BUFFER
To make 1 Liter

1. Dissolve the following in ~800mL18 MΩ dH2O.  May need to heat a little to dissolve completely.
80g NaCl  14.4g Na2HPO4–7H2O (dibasic)
  2g KCl    2.4g KH2PO4 (monobasic)
2. Adjust pH to 7.4 with HCl or NaOH.
3. Raise volume to 1 liter.
4. Transfer to a 1L bottle, label and autoclave.

Reference

10X, 20X TRIS Buffer Recipes

10X Tris-HCl (0.5M Tris Base, pH7.6):
      Trizma Base ---------------------------------- 61 g
      Distilled water ------------------------------- 1000 ml
      Adjust pH 7.6 using concentrated HCl
       Store this solution at room temperature. Dilute 1:10 with distilled water before use
and adjust pH if necessary.
 
20X Tris-HCl (1M Tris Base, pH7.6):
      Trizma Base ---------------------------------- 122 g
      Distilled water ------------------------------- 1000 ml
      Adjust pH 7.6 using concentrated HCl
      Store this solution at room temperature. Dilute 1:20 with distilled water before use
and adjust pH if necessary.
 
10X Tris-HCl-Tween 20 (0.5M Tris Base, 0.5% Tween 20, pH7.6):
      Trizma Base ---------------------------------- 61 g
      Distilled water ------------------------------- 1000 ml
      Adjust pH 7.6 using concentrated HCl and then add 5 ml of Tween 20.
       Store this solution at room temperature. Dilute 1:10 with distilled water before use
and adjust pH if necessary.
 
Reference

10% SDS Recipe

10% SDS  Recipe

1L:  

100g SDS into 1 L, heat to 68C for solubility. pH ~6.6.

50X TAE DNA Electrophoresis Buffer Recipe

 TAE DNA Electrophoresis Buffer (50 X)

     (2 M Tris, 50 mM EDTA)    
     
     2 L
     484 g Tris
     114.2 ml glacial acetic acid
     200 ml 0.5 M EDTA 8.0  
To make 1x TAE 20 L, add 400 ml 50X buffer into 19.6 L ddH2O

Reference

0.5M EDTA pH 8.0 Recipe

EDTA 0.5 M (pH8.0)

    0.5M, 1L:  148 g EDTA
                       + ~30-40 g NaOH to adjust pH
                       (or 186 g EDTA-Na.2H2O + ~20 g NaOH)
   Note: pH adjusted by NaOH is essential for solubility. Autoclavable

Reference

1 M TRIS Buffer Recipe

1 M Tris-HCl Buffers
 
pH        Volume (L)              TrisBase (g)         HCl (ml)
pH 7.0  2                             242.2                  150-155
pH 7.5  2                             242.2                  120-125
pH 8.0  2                             242.2                   80-85

Autoclavable

Reference

0.5 x TBE Buffer Recipe

0.5 x tbe buffer recipe


-108 g Tris base
-55 g Boric acid
-40 ml 0.5 M EDTA pH 8.0
-H2O to 2 L

Place the flask on a magnetic stirrer until completely dissolved. This results in a 5X concentrated
stock of TBE Buffer. Dilute the 2L of 5X stock by 10 in distilled water (add 18 L or 4.5 gallons of
distilled water). Mix well. The buffer is ready for use.

Reference