50X TAE DNA Electrophoresis Buffer Recipe

 TAE DNA Electrophoresis Buffer (50 X)

     (2 M Tris, 50 mM EDTA)    
     
     2 L
     484 g Tris
     114.2 ml glacial acetic acid
     200 ml 0.5 M EDTA 8.0  
To make 1x TAE 20 L, add 400 ml 50X buffer into 19.6 L ddH2O

Reference