10X PBS BUFFER
To make 1 Liter
1. Dissolve the following in ~800mL18 MΩ dH2O. May need to heat a little to dissolve completely.
80g NaCl 14.4g Na2HPO4–7H2O (dibasic)
2g KCl 2.4g KH2PO4 (monobasic)
2. Adjust pH to 7.4 with HCl or NaOH.
3. Raise volume to 1 liter.
4. Transfer to a 1L bottle, label and autoclave.
Reference
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