10X, 20X TRIS Buffer Recipes

10X Tris-HCl (0.5M Tris Base, pH7.6):
      Trizma Base ---------------------------------- 61 g
      Distilled water ------------------------------- 1000 ml
      Adjust pH 7.6 using concentrated HCl
       Store this solution at room temperature. Dilute 1:10 with distilled water before use
and adjust pH if necessary.
 
20X Tris-HCl (1M Tris Base, pH7.6):
      Trizma Base ---------------------------------- 122 g
      Distilled water ------------------------------- 1000 ml
      Adjust pH 7.6 using concentrated HCl
      Store this solution at room temperature. Dilute 1:20 with distilled water before use
and adjust pH if necessary.
 
10X Tris-HCl-Tween 20 (0.5M Tris Base, 0.5% Tween 20, pH7.6):
      Trizma Base ---------------------------------- 61 g
      Distilled water ------------------------------- 1000 ml
      Adjust pH 7.6 using concentrated HCl and then add 5 ml of Tween 20.
       Store this solution at room temperature. Dilute 1:10 with distilled water before use
and adjust pH if necessary.
 
Reference