0.5 x TBE Buffer Recipe

0.5 x tbe buffer recipe


-108 g Tris base
-55 g Boric acid
-40 ml 0.5 M EDTA pH 8.0
-H2O to 2 L

Place the flask on a magnetic stirrer until completely dissolved. This results in a 5X concentrated
stock of TBE Buffer. Dilute the 2L of 5X stock by 10 in distilled water (add 18 L or 4.5 gallons of
distilled water). Mix well. The buffer is ready for use.

Reference