Saturday, 24 December 2011
0.2M EGTA stock solution for 10ml
0.2M EGTA stock solution for 10ml
Mix on magnetic stirrer:
8ml Milli Q water
0.7608g EGTA{ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) Sigma E-4378 LOT 111K5411 FW 380,4}
Adjust to pH8 with 10N NaOH otherwise it won't disolve (be careful EGTA is an acid)
Adjust volume to 10ml with Milli Q water
Store at 4C
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