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Saturday 24 December 2011

0.2M EGTA stock solution for 10ml

0.2M EGTA stock solution for 10ml Mix on magnetic stirrer: 8ml Milli Q water 0.7608g EGTA{ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) Sigma E-4378 LOT 111K5411 FW 380,4} Adjust to pH8 with 10N NaOH otherwise it won't disolve (be careful EGTA is an acid) Adjust volume to 10ml with Milli Q water Store at 4C

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