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Saturday 24 December 2011

Ca-Mg-VBS (veronal buffered saline) for 25ml

Ca-Mg-VBS (veronal buffered saline) for 25ml (for serum dilution)[2.5mM Ca2+, 0.7mM Mg2+, 0.05% Tween 20, 5%BSA, VBS] Mix on magnetic stirrer: 5ml 5x VBS 250ul Ca2+ Mg2+ stock solution [0.25M Ca2+, 0.07M Mg2+] 1.25g BSA {Sigma, No.:A3059-100G, Albumin, bovine serum, Fraction V} 12.5ul Tween 20 {Sigma No.:P-1379, Polyoxylethylene-sorbitan monolaurate (Tween 20)} Adjust volume to 25ml with Milli Q water Filtrate though an 0.45um filter Aliquot 1ml/eppendorf Store at -20C

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