Saturday, 24 December 2011
Ca-Mg-VBS (veronal buffered saline) for 25ml
Ca-Mg-VBS (veronal buffered saline) for 25ml (for serum dilution)[2.5mM Ca2+, 0.7mM Mg2+, 0.05% Tween 20, 5%BSA, VBS]
Mix on magnetic stirrer:
5ml 5x VBS
250ul Ca2+ Mg2+ stock solution [0.25M Ca2+, 0.07M Mg2+]
1.25g BSA {Sigma, No.:A3059-100G, Albumin, bovine serum, Fraction V}
12.5ul Tween 20 {Sigma No.:P-1379, Polyoxylethylene-sorbitan monolaurate (Tween 20)}
Adjust volume to 25ml with Milli Q water
Filtrate though an 0.45um filter
Aliquot 1ml/eppendorf
Store at -20C
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