Saturday 24 December 2011
PBS-Tween BSA for 300ml [0.05% Tween 20, 5%BSA, 0.05% azide, PBS]
PBS-Tween BSA for 300ml [0.05% Tween 20, 5%BSA, 0.05% azide, PBS]
Mix on magnetic stirrer:
300ml PBS-Tween
15g BSA {Sigma, No.:A3059-100G, Albumin, bovine serum, Fraction V}
1.5ml Sodium azide (from 10% NaN3 stock solution)
Filtrate through an 0.45um filter
Aliquot 50ml/falcon
Store at 4°C
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