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Saturday 24 December 2011

Qiagen RNeasy Plant RNA Isolation


RNA isolation
RNeasy Kits are designed to isolate total RNA from small quantities of starting material. They provide a fast and simple method for the preparation of up to 100 µg total RNA from animal cells and tissues, bacteria, and yeast (RNeasy Mini Kits) or plant cells and tissues and filamentous fungi (RNeasy Plant Mini Kits). RNeasy Plant Mini Kits are fast and avoid tedious methods, such as CsCl step-gradient ultracentrifugation and alcohol precipitation steps, or methods involving the use of toxic substances such as phenol and/or chloroform. The purified RNA is ready for use in standard downstream applications such as RT-PCR, Northern, dot, and slot blotting, poly A+ RNA selection, primer extension, RNase and S1 nuclease protection, cDNA synthesis, differential display, expression-array and expression-chip analysis.

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