RIPA Lysis Buffer Recipe : RIPA buffer for mammalian cell lysis:

RIPA buffer for mammalian cell lysis:
50mM Tris-cl pH 7.4
150mM NaCl
1% NP40
0.25% Na-deoxycholate
1mM PMSF
1x Roche complete mini protease inhibitor cocktail
1x Pierce phosphatase inhibitor cocktail

Stock solutions used:
100mM Tris-Cl pH 7.4, 300mM NaCl
10% NP40 in water
10% Na-deoxycholate in water (stored at RT in dark)
200mM PMSF in isopropanol
Roche complete protease inhibitor cocktail tablets (cat# 04 693 124 001)
Pierce phosphatase inhibitor cocktail (100x) (cat # 78420)
For 10ml of lysis buffer:
5ml  100mM Tris-Cl pH 7.4, 300mM NaCl
1ml  10% NP40 in water
3.65ml  water
250ul  10% Na-deoxycholate in water
1 tablet * Roche complete protease inhibitor cocktail
100ul*  Pierce phosphatase inhibitor cocktail
50ul*  200mM PMSF in isopropanol

*add at time of use. PMSF is only good for ~30minutes after dilution into buffer.

Reference [pdf]