ELISA blocking-buffer for 10ml [0.05% Tween 20, 5%BSA, 0.05% azide, PBS]
- Mix on magnetic stirrer:
- 10ml PBS-Tween
- 100mg BSA {Sigma, No.:A3059-100G, Albumin, bovine serum, Fraction V}
- 50ul Sodium azide (from 10% NaN3 stock solution)
- Store at +4C
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