International Scholarships and Financial Aid

Saturday, 24 December 2011

ELISA blocking-buffer for 10ml


ELISA blocking-buffer for 10ml [0.05% Tween 20, 5%BSA, 0.05% azide, PBS]
  • Mix on magnetic stirrer:
    • 10ml PBS-Tween
    • 100mg BSA {Sigma, No.:A3059-100G, Albumin, bovine serum, Fraction V}
    • 50ul Sodium azide (from 10% NaN3 stock solution)
  • Store at +4C

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