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Saturday, 24 December 2011
GRAM STAIN PROTOCOL - fecal smear to observe the bacterial microflora
This technique is used to stain a slide such as a fecal smear to observe the bacterial microflora present based on their gram stain reaction.
“Heat-fix” the slide with the specimen by passing it over a heat source, such as a
flame, several times using a forceps. The slide should be passed very quickly through
the flame and not be heated excessively. Place slide on the staining tray.
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