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Saturday, 24 December 2011

Mg-EGTA-VBS solution for 10ml

Mg-EGTA-VBS solution for 10ml (dilute serum 5x with it)[2.5mM Mg2+, 6.2mM EGTA, 5%BSA, 0.05% Tween 20, VBS] Mix on magnetic stirrer: 2ml 5x VBS 310ul 0.2M EGTA 25ul 1M MgCl2 stock solution 0.5g BSA {Sigma, No.:A3059-100G, Albumin, bovine serum, Fraction V} 50ul 10% Tween 20 {Sigma No.:P-1379, Polyoxylethylene-sorbitan monolaurate (Tween 20)} Adjust volume to 10ml with Milli Q water Filtrate though an 0.45um filter Aliquot 1ml/eppendorf Store at -20C

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