Saturday 24 December 2011
Mg-EGTA-VBS solution for 10ml
Mg-EGTA-VBS solution for 10ml (dilute serum 5x with it)[2.5mM Mg2+, 6.2mM EGTA, 5%BSA, 0.05% Tween 20, VBS]
Mix on magnetic stirrer:
2ml 5x VBS
310ul 0.2M EGTA
25ul 1M MgCl2 stock solution
0.5g BSA {Sigma, No.:A3059-100G, Albumin, bovine serum, Fraction V}
50ul 10% Tween 20 {Sigma No.:P-1379, Polyoxylethylene-sorbitan monolaurate (Tween 20)}
Adjust volume to 10ml with Milli Q water
Filtrate though an 0.45um filter
Aliquot 1ml/eppendorf
Store at -20C
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