5x Veronal buffer for 500ml (5xVBS) Recipe

5x Veronal buffer for 500ml (5xVBS) [727mM NaCl, 9.12mM Na-diethyl-barbiturate (C8H11O3N2Na), 15.63mM 5,5’-diethyl barbiture acid (C8H12N2O3), pH7.3] Make solution 'A': 0.94g Na-diethyl-barbiturate (C8H11O3N2Na){5,5-Diathylbarbitursaure. Na-salz Serva 18797; MW:206.2} 21.25 g NaCl {Reanal, No.:2464-1-22-38, Nátrium-klorid, Ph. Eur.5; MW:58.44} ~300ml Milli Q water Make solution 'B': 1.44g 5,5’- diethyl barbiture acid {5,5-dietil-barbitursav Reanal 04049; MW:184.2} ~100ml Milli Q water Boil it otherwise it won’t dissolve, then cool it down Mix solution ‘A’ and ‘B’ set pH to 7.3 with app. 50-70μl 10N NaOH Fill up until 500ml with Milli Q water, then store it at +4C