This section outlines the protocol for single-molecule amplification within the acrylamide gel. The protocol given here may differ in specifics from what is presented here, but reflects what we are doing currently (Summer 2003). We have tried to include excessive detail here but let us know if anything is unclear. The general steps are:
1. Cast acrylamide gels
2. Diffuse in PCR reagents
3. PCR amplification
4. Slide clean-up
Further Protocol
No comments:
Post a Comment