Lysis buffer: RIPA or NP40 for Immunoprecipitation

Lysis buffer.

The choice of lysis buffer depends on what kind of IP you are doing.

RIPA buffer gives the lowest background, but can denature some kinases. It also has the potential to disrupt protein:protein interactions.

NP40 buffer (EVF) is less denaturing, but gives a higher background. It is less likely to inhibit kinase activity and disrupt protein complexes.

RIPA Buffer recipe

Further Buffer Information