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Saturday, 24 December 2011

1x PBS Recipe for 1 liter[137mM NaCl, 2.7mM KCl, 8mM Na2HPO4, 1.46mM KH2PO4]

Dissolve in 800 ml distilled water: 8 g NaCl {Reanal, No.:2464-1-22-38, Nátrium-klorid, Ph. Eur.5; MW:58.44} 0.2 g KCl {Reanal, No.:18050-1-01-38, Kálium-klorid, a.r.; MW: 74.56} 1.424 g Na2HPO4*2H2O {Reanal, No.:08973-1-01-38, di-Nátrium-hidrogén-foszfát 2-hidrát, a.r.; MW:177,99} 0.2 g KH2PO4 {Reanal, No.:17890-01-38, Kálium-dihidrogén-foszfát, a.r.; MW:136.09} Adjust volume to 1 liter with H2O Filtrate trough an 0.22um filter or sterilize by autoclave Store at room temperature

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