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Tuesday 13 December 2011

How to make 1x pbs from 10x pbs

If you have tablets then make the 10x pbs solution according the the manufacturer's instructions.

Than use the formula C1V1=C2V2 to make the dilution for your required volume.
C=Concentration
V=Volum


Make 20 ml of 1X PBS buffer from a 10X stock.


        10X stock *  X  =  1X stock *  20 ml

                     X = (1X stock *  20 ml) / 10X stock

                     X = 2.0 ul 10X stock
                       +18.0 ul of H2O

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