Here we have searched and compiled a list of methods used by various college and universities for the isolation of acid phosphatase.
Purification of Acid Phosphatase from Wheat Germ
There are three major steps involved in the purification of macromolecules. First, you must disrupt the cell you are using as a source. Second, you must selectively purify the macromolecule away from contaminating molecules. Third, you must both prevent degradation of the macromolecule during the procedure and you must preserve the native structure of the macromolecule to be purified. In the purification of proteins, you want to separate that protein from other “contaminating” proteins, and you want to purify it in its native conformation with retention of enzymatic activity.
Further Details
Purification of Acid Phosphatase from Wheat Germ
There are three major steps involved in the purification of macromolecules. First, you must disrupt the cell you are using as a source. Second, you must selectively purify the macromolecule away from contaminating molecules. Third, you must both prevent degradation of the macromolecule during the procedure and you must preserve the native structure of the macromolecule to be purified. In the purification of proteins, you want to separate that protein from other “contaminating” proteins, and you want to purify it in its native conformation with retention of enzymatic activity.
Further Details
Purification and Partial Characterization of an Acid Phosphatase fromSpirodela oligorrhiza and Its Affinity for Selected Organophosphate Pesticides
National Research Council, and National Exposure Research Laboratory, U.S. Environmental Protection Agency, 960 College Station Road, Athens, Georgia 30605http://pubs.acs.org/doi/abs/10.1021/jf040329u
Isolation and partial characterization of an acid phosphatase
from Artemisia vulgaris pollen extract
TANJA D. ]IRKOVI]*#, MARIJA DJ. GAVROVI]-JANKULOVI]* #, MIRJANA N. BUKILICA**,
LJUBA MANDI]*#, SPOMENKA Z. PETROVI]*** and RATKO M. JANKOV*
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